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Title: MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense

Abstract

Many methylotrophs, microorganisms that consume carbon compounds lacking carbon–carbon bonds, use two different systems to oxidize methanol for energy production and biomass accumulation. The MxaFI methanol dehydrogenase (MDH) contains calcium in its active site, while the XoxF enzyme contains a lanthanide in its active site. The genes encoding the MDH enzymes are differentially regulated by the presence of lanthanides. In this study, we found that the histidine kinase MxaY controls the lanthanide-mediated switch in Methylomicrobium buryatense 5GB1C. MxaY controls the transcription of genes encoding MxaFI and XoxF at least partially by controlling the transcript levels of the orphan response regulator MxaB. We identify a constitutively active version of MxaY, and identify the mutated residue that may be involved in lanthanide sensing. Finally, we find evidence to suggest that tight control of active MDH production is required for wild-type growth rates.

Authors:
 [1];  [1];  [1]
  1. Univ. of Washington, Seattle, WA (United States)
Publication Date:
Research Org.:
Univ. of Washington, Seattle, WA (United States). Dept. of Chemical Engineering
Sponsoring Org.:
USDOE
OSTI Identifier:
1362115
Grant/Contract Number:  
AR0000350
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PeerJ
Additional Journal Information:
Journal Volume: 4; Journal ID: ISSN 2167-8359
Publisher:
PeerJ Inc.
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; methanol dehydrogenase; XoxF; lanthanides; MxaY; MxaFI

Citation Formats

Chu, Frances, Beck, David A. C., and Lidstrom, Mary E.. MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense. United States: N. p., 2016. Web. doi:10.7717/peerj.2435.
Chu, Frances, Beck, David A. C., & Lidstrom, Mary E.. MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense. United States. doi:10.7717/peerj.2435.
Chu, Frances, Beck, David A. C., and Lidstrom, Mary E.. Wed . "MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense". United States. doi:10.7717/peerj.2435. https://www.osti.gov/servlets/purl/1362115.
@article{osti_1362115,
title = {MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense},
author = {Chu, Frances and Beck, David A. C. and Lidstrom, Mary E.},
abstractNote = {Many methylotrophs, microorganisms that consume carbon compounds lacking carbon–carbon bonds, use two different systems to oxidize methanol for energy production and biomass accumulation. The MxaFI methanol dehydrogenase (MDH) contains calcium in its active site, while the XoxF enzyme contains a lanthanide in its active site. The genes encoding the MDH enzymes are differentially regulated by the presence of lanthanides. In this study, we found that the histidine kinase MxaY controls the lanthanide-mediated switch in Methylomicrobium buryatense 5GB1C. MxaY controls the transcription of genes encoding MxaFI and XoxF at least partially by controlling the transcript levels of the orphan response regulator MxaB. We identify a constitutively active version of MxaY, and identify the mutated residue that may be involved in lanthanide sensing. Finally, we find evidence to suggest that tight control of active MDH production is required for wild-type growth rates.},
doi = {10.7717/peerj.2435},
journal = {PeerJ},
number = ,
volume = 4,
place = {United States},
year = {Wed Sep 07 00:00:00 EDT 2016},
month = {Wed Sep 07 00:00:00 EDT 2016}
}

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Cited by: 6 works
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Works referenced in this record:

Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009

  • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
  • Nature Methods, Vol. 6, Issue 5, p. 343-345
  • DOI: 10.1038/nmeth.1318