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Title: Final report for project "Effects of Low-Dose Irradiation on NFkB Signaling Networks and Mitochondria"

Abstract

Low dose ionizing radiation effects are difficult to study in human population because of the numerous confounding factors such as genetic and lifestyle differences. Research in mammalian model systems and in vitro is generally used in order to overcome this difficulty. In this program project three projects have joined together to investigate effects of low doses of ionizing radiation. These are doses at and below 10 cGy of low linear energy transfer ionizing radiation such as X-ray and gamma rays. This project was focused on cellular signaling associated with nuclear factor kappa B (NFkB) and mitochondria - subcellular organelles critical for cell aging and aging-like changes induced by ionizing radiation. In addition to cells in culture this project utilized animal tissues accumulated in a radiation biology tissue archive housed at Northwestern University (http://janus.northwestern.edu/janus2/index.php). Major trust of Project 1 was to gather all of the DoE sponsored irradiated animal (mouse, rat and dog) data and tissues under one roof and investigate mitochondrial DNA changes and micro RNA changes in these samples. Through comparison of different samples we were trying to delineate mitochondrial DNA quantity alterations and micro RNA expression differences associated with different doses and dose rates of radiation. Historic animalmore » irradiation experiments sponsored by DoE were done in several national laboratories and universities between 1950’s and 1990’s; while these experiments were closed data and tissues were released to Project 1. Project 2 used cells in culture to investigate effects that low doses or radiation have on NFκB and its target genes manganese superoxide dismutase (MnSOD) and genes involved in cell cycle: Cyclins (B1 and D1) and cyclin dependent kinases (CDKs). Project 3 used cells in culture such as “normal” human cells (breast epithelial cell line MCF10A cells and skin keratinocyte cells HK18) and mouse embryo fibroblast (mef) cells to focus on role of NFkB protein and several other proteins such as survivin (BIRC5) in radiation dependent regulation of tumor necrosis factor alpha (TNFα) and its downstream signaling.« less

Authors:
ORCiD logo [1];  [2];  [3]
  1. Northwestern Univ., Evanston, IL (United States)
  2. Univ. of Chicago, IL (United States)
  3. Univ. of California, Davis, CA (United States)
Publication Date:
Research Org.:
Northwestern Univ., Evanston, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1362082
Report Number(s):
DOE-NU-SC0001271
DOE Contract Number:  
SC0001271
Resource Type:
Technical Report
Resource Relation:
Related Information: http://janus.northwestern.edu/janus2/index.php
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 61 RADIATION PROTECTION AND DOSIMETRY; low dose ionizing radiation; animal models; data and tissue archives; nuclear factor kappa B

Citation Formats

Woloschak, Gayle E, Grdina, David, and Li, Jian-Jian. Final report for project "Effects of Low-Dose Irradiation on NFkB Signaling Networks and Mitochondria". United States: N. p., 2017. Web. doi:10.2172/1362082.
Woloschak, Gayle E, Grdina, David, & Li, Jian-Jian. Final report for project "Effects of Low-Dose Irradiation on NFkB Signaling Networks and Mitochondria". United States. doi:10.2172/1362082.
Woloschak, Gayle E, Grdina, David, and Li, Jian-Jian. Mon . "Final report for project "Effects of Low-Dose Irradiation on NFkB Signaling Networks and Mitochondria"". United States. doi:10.2172/1362082. https://www.osti.gov/servlets/purl/1362082.
@article{osti_1362082,
title = {Final report for project "Effects of Low-Dose Irradiation on NFkB Signaling Networks and Mitochondria"},
author = {Woloschak, Gayle E and Grdina, David and Li, Jian-Jian},
abstractNote = {Low dose ionizing radiation effects are difficult to study in human population because of the numerous confounding factors such as genetic and lifestyle differences. Research in mammalian model systems and in vitro is generally used in order to overcome this difficulty. In this program project three projects have joined together to investigate effects of low doses of ionizing radiation. These are doses at and below 10 cGy of low linear energy transfer ionizing radiation such as X-ray and gamma rays. This project was focused on cellular signaling associated with nuclear factor kappa B (NFkB) and mitochondria - subcellular organelles critical for cell aging and aging-like changes induced by ionizing radiation. In addition to cells in culture this project utilized animal tissues accumulated in a radiation biology tissue archive housed at Northwestern University (http://janus.northwestern.edu/janus2/index.php). Major trust of Project 1 was to gather all of the DoE sponsored irradiated animal (mouse, rat and dog) data and tissues under one roof and investigate mitochondrial DNA changes and micro RNA changes in these samples. Through comparison of different samples we were trying to delineate mitochondrial DNA quantity alterations and micro RNA expression differences associated with different doses and dose rates of radiation. Historic animal irradiation experiments sponsored by DoE were done in several national laboratories and universities between 1950’s and 1990’s; while these experiments were closed data and tissues were released to Project 1. Project 2 used cells in culture to investigate effects that low doses or radiation have on NFκB and its target genes manganese superoxide dismutase (MnSOD) and genes involved in cell cycle: Cyclins (B1 and D1) and cyclin dependent kinases (CDKs). Project 3 used cells in culture such as “normal” human cells (breast epithelial cell line MCF10A cells and skin keratinocyte cells HK18) and mouse embryo fibroblast (mef) cells to focus on role of NFkB protein and several other proteins such as survivin (BIRC5) in radiation dependent regulation of tumor necrosis factor alpha (TNFα) and its downstream signaling.},
doi = {10.2172/1362082},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jun 12 00:00:00 EDT 2017},
month = {Mon Jun 12 00:00:00 EDT 2017}
}

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