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Title: Ultrasensitive biochemical sensing device and method of sensing analytes

Abstract

Systems and methods biochemically sense a concentration of a ligand using a sensor having a substrate having a metallic nanoparticle array formed onto a surface of the substrate. A light source is incident on the surface. A matrix is deposited over the nanoparticle array and contains a protein adapted to binding the ligand. A detector detects s-polarized and p-polarized light from the reflective surface. Spacing of nanoparticles in the array and wavelength of light are selected such that plasmon resonance occurs with an isotropic point such that -s and -p polarizations of the incident light result in substantially identical surface Plasmon resonance, wherein binding of the ligand to the protein shifts the resonance such that differences between the -S and -P polarizations give in a signal indicative of presence of the ligand.

Inventors:
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1361542
Patent Number(s):
9,671,327
Application Number:
13/422,718
Assignee:
The Regents of the University of Colorado, A Body Corporate ANL
DOE Contract Number:
AC02-06CH11357
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 16
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Pinchuk, Anatoliy. Ultrasensitive biochemical sensing device and method of sensing analytes. United States: N. p., 2017. Web.
Pinchuk, Anatoliy. Ultrasensitive biochemical sensing device and method of sensing analytes. United States.
Pinchuk, Anatoliy. 2017. "Ultrasensitive biochemical sensing device and method of sensing analytes". United States. doi:. https://www.osti.gov/servlets/purl/1361542.
@article{osti_1361542,
title = {Ultrasensitive biochemical sensing device and method of sensing analytes},
author = {Pinchuk, Anatoliy},
abstractNote = {Systems and methods biochemically sense a concentration of a ligand using a sensor having a substrate having a metallic nanoparticle array formed onto a surface of the substrate. A light source is incident on the surface. A matrix is deposited over the nanoparticle array and contains a protein adapted to binding the ligand. A detector detects s-polarized and p-polarized light from the reflective surface. Spacing of nanoparticles in the array and wavelength of light are selected such that plasmon resonance occurs with an isotropic point such that -s and -p polarizations of the incident light result in substantially identical surface Plasmon resonance, wherein binding of the ligand to the protein shifts the resonance such that differences between the -S and -P polarizations give in a signal indicative of presence of the ligand.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 2017,
month = 6
}

Patent:

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  • By measuring two or more physical parameters of a thin sensing film which are altered when exposed to chemicals, more effective discrimination between chemicals can be achieved. In using more than one sensor, the sensors are preferably integrated on the same substrate so that they may measure the same thin film. Even more preferably, the sensors are provided orthogonal to one another so that they may measure the same portion of the thin film. These provisions reduce problems in discrimination arising from variations in thin films.
  • By measuring two or more physical parameters of a thin sensing film which are altered when exposed to chemicals, more effective discrimination between chemicals can be achieved. In using more than one sensor, the sensors are preferably integrated on the same substrate so that they may measure the same thin film. Even more preferably, the sensors are provided orthogonal to one another so that they may measure the same portion of the thin film. These provisions reduce problems in discrimination arising from variations in thin films.
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