skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin

Abstract

We provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination. Se-B SA was chosen as the model system for its high affinity between biotin and streptavidin where the sulfur atom in the biotin molecule (C 10H 16N 2O 3S) is substituted with selenium. The dataset was collected at three different transmissions (100, 50, and 10%) using a serial sample chamber setup which allows for two sample chambers, a front chamber and a back chamber, to operate simultaneously. Diffraction patterns from Se-B SA were recorded to a resolution of 1.9 Å. The dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) and also on LCLS compute nodes as a resource for research and algorithm development.

Authors:
 [1]; ORCiD logo [2];  [1];  [3];  [1];  [4];  [1]; ORCiD logo [1];  [1];  [1];  [1];  [1];  [5];  [1];  [6];  [1];  [1];  [1];  [1];  [1] more »;  [1];  [5];  [1];  [7];  [1] « less
  1. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst. Biosciences Division. Stanford Synchrotron Radiation Lightsource
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst. Biosciences Division
  4. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford PULSE Inst.
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source. Biosciences Division
  6. SLAC National Accelerator Lab., Menlo Park, CA (United States). Biosciences Division
  7. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource; Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Laboratory Directed Research and Development (LDRD) Program; National Inst. of Health (NIH) (United States)
Contributing Org.:
Univ. of California, San Francisco, CA (United States)
OSTI Identifier:
1361134
Grant/Contract Number:  
AC02-76SF00515; P41GM103393; P41RR001209
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Data
Additional Journal Information:
Journal Volume: 4; Journal ID: ISSN 2052-4463
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 36 MATERIALS SCIENCE; Nanocrystallography; Macromolecules and clusters; Imaging; Biological physics

Citation Formats

Yoon, Chun Hong, DeMirci, Hasan, Sierra, Raymond G., Dao, E. Han, Ahmadi, Radman, Aksit, Fulya, Aquila, Andrew L., Batyuk, Alexander, Ciftci, Halilibrahim, Guillet, Serge, Hayes, Matt J., Hayes, Brandon, Lane, Thomas J., Liang, Meng, Lundström, Ulf, Koglin, Jason E., Mgbam, Paul, Rao, Yashas, Rendahl, Theodore, Rodriguez, Evan, Zhang, Lindsey, Wakatsuki, Soichi, Boutet, Sébastien, Holton, James M., and Hunter, Mark S. Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin. United States: N. p., 2017. Web. doi:10.1038/sdata.2017.55.
Yoon, Chun Hong, DeMirci, Hasan, Sierra, Raymond G., Dao, E. Han, Ahmadi, Radman, Aksit, Fulya, Aquila, Andrew L., Batyuk, Alexander, Ciftci, Halilibrahim, Guillet, Serge, Hayes, Matt J., Hayes, Brandon, Lane, Thomas J., Liang, Meng, Lundström, Ulf, Koglin, Jason E., Mgbam, Paul, Rao, Yashas, Rendahl, Theodore, Rodriguez, Evan, Zhang, Lindsey, Wakatsuki, Soichi, Boutet, Sébastien, Holton, James M., & Hunter, Mark S. Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin. United States. doi:10.1038/sdata.2017.55.
Yoon, Chun Hong, DeMirci, Hasan, Sierra, Raymond G., Dao, E. Han, Ahmadi, Radman, Aksit, Fulya, Aquila, Andrew L., Batyuk, Alexander, Ciftci, Halilibrahim, Guillet, Serge, Hayes, Matt J., Hayes, Brandon, Lane, Thomas J., Liang, Meng, Lundström, Ulf, Koglin, Jason E., Mgbam, Paul, Rao, Yashas, Rendahl, Theodore, Rodriguez, Evan, Zhang, Lindsey, Wakatsuki, Soichi, Boutet, Sébastien, Holton, James M., and Hunter, Mark S. Tue . "Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin". United States. doi:10.1038/sdata.2017.55. https://www.osti.gov/servlets/purl/1361134.
@article{osti_1361134,
title = {Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin},
author = {Yoon, Chun Hong and DeMirci, Hasan and Sierra, Raymond G. and Dao, E. Han and Ahmadi, Radman and Aksit, Fulya and Aquila, Andrew L. and Batyuk, Alexander and Ciftci, Halilibrahim and Guillet, Serge and Hayes, Matt J. and Hayes, Brandon and Lane, Thomas J. and Liang, Meng and Lundström, Ulf and Koglin, Jason E. and Mgbam, Paul and Rao, Yashas and Rendahl, Theodore and Rodriguez, Evan and Zhang, Lindsey and Wakatsuki, Soichi and Boutet, Sébastien and Holton, James M. and Hunter, Mark S.},
abstractNote = {We provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination. Se-B SA was chosen as the model system for its high affinity between biotin and streptavidin where the sulfur atom in the biotin molecule (C10H16N2O3S) is substituted with selenium. The dataset was collected at three different transmissions (100, 50, and 10%) using a serial sample chamber setup which allows for two sample chambers, a front chamber and a back chamber, to operate simultaneously. Diffraction patterns from Se-B SA were recorded to a resolution of 1.9 Å. The dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) and also on LCLS compute nodes as a resource for research and algorithm development.},
doi = {10.1038/sdata.2017.55},
journal = {Scientific Data},
number = ,
volume = 4,
place = {United States},
year = {Tue Apr 25 00:00:00 EDT 2017},
month = {Tue Apr 25 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share: