Characterization of chemically modified hyperthermophilic enzymes for chemical syntheses and bioremediation reactions. 1998 annual progress report
- Oak Ridge National Lab., TN (US)
- Univ. of Georgia, Athens, GA (US)
'Remediation processes frequently involve species possessing limited solubility in water. The authors are interested in novel strategies that use molecularly-engineered enzymes with enhanced activity and stability for the remediation of recalcitrant compounds in organic media. Organic biocatalysis may be motivated by the nature of the substrate itself, or by augmented mass transport, ease of product recovery, or novel reaction pathways afforded by the organic solvent. However, naturally-occurring enzymes are usually subject to quite limited activity and stability in such organic environments. The objective of the current work is to gain a fundamental understanding of the molecular and catalytic properties of enzymes that have been chemically-modified so that they are catalytically-active and chemically-thermally-stable in organic solvents. The premise for this study is that highly stable enzymes which are catalytically active in both water and in a range of organic solvents are optimally suited for bioremediation where substrates of interest are more soluble and may be processed with greater specificity in nonaqueous solvents. This bioprocessing system will be assessed using PCB''s, DNAPL''s and PAH''s. As of the second of year of a 3-year project, the authors have obtained promising results for both the demonstration of the proposed remediation strategy and fundamental understanding of the enzymatic catalysis in organic media. In one of the efforts, ligninase (LiP) was modified by poly(ethylene glycol) (PEG) and examined for the degradation of pentachlorophenol (PCP) in water-solvent mixtures. In the other efforts, basic catalytic behaviors of the chemically modified hyperthermophilic metalloenzymes including ferredoxin, hydrogenase and aldehyde oxidoreductase were examined in organic solvents.'
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE Office of Environmental Management (EM), Office of Science and Risk Policy
- OSTI ID:
- 13592
- Report Number(s):
- EMSP-55033-98; ON: DE00013592
- Country of Publication:
- United States
- Language:
- English
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