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Title: Methods for producing extracted and digested products from pretreated lignocellulosic biomass

Abstract

Methods for producing extracted and digested products from pretreated lignocellulosic biomass are provided. The methods include converting native cellulose I.sub..beta. to cellulose III.sub.I by pretreating the lignocellulosic biomass with liquid ammonia under certain conditions, and performing extracting or digesting steps on the pretreated/converted lignocellulosic biomass.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY, East Lansing, MI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1357532
Patent Number(s):
9,650,657
Application Number:
13/642,052
Assignee:
BOARD OF TRUSTEES OF MICHIGAN STATE UNIVERSITY CHO
DOE Contract Number:
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Apr 19
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Chundawat, Shishir, Sousa, Leonardo Da Costa, Cheh, Albert M., Balan;, Venkatesh, and Dale, Bruce. Methods for producing extracted and digested products from pretreated lignocellulosic biomass. United States: N. p., 2017. Web.
Chundawat, Shishir, Sousa, Leonardo Da Costa, Cheh, Albert M., Balan;, Venkatesh, & Dale, Bruce. Methods for producing extracted and digested products from pretreated lignocellulosic biomass. United States.
Chundawat, Shishir, Sousa, Leonardo Da Costa, Cheh, Albert M., Balan;, Venkatesh, and Dale, Bruce. Tue . "Methods for producing extracted and digested products from pretreated lignocellulosic biomass". United States. doi:. https://www.osti.gov/servlets/purl/1357532.
@article{osti_1357532,
title = {Methods for producing extracted and digested products from pretreated lignocellulosic biomass},
author = {Chundawat, Shishir and Sousa, Leonardo Da Costa and Cheh, Albert M. and Balan;, Venkatesh and Dale, Bruce},
abstractNote = {Methods for producing extracted and digested products from pretreated lignocellulosic biomass are provided. The methods include converting native cellulose I.sub..beta. to cellulose III.sub.I by pretreating the lignocellulosic biomass with liquid ammonia under certain conditions, and performing extracting or digesting steps on the pretreated/converted lignocellulosic biomass.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 16 00:00:00 EDT 2017},
month = {Tue May 16 00:00:00 EDT 2017}
}

Patent:

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  • A process is provided comprising subjecting a quantity of plant biomass fibers to a pretreatment to cause at least a portion of lignin contained within each fiber to move to an outer surface of said fiber, wherein a quantity of pretreated tacky plant biomass fibers is produced; and densifying the quantity of pretreated tacky plant biomass fibers to produce one or more densified biomass particulates, wherein said biomass fibers are densified without using added binder.
  • The present invention is an improved method of making a partially modified PCR product from a DNA fragment with a polymerase chain reaction (PCR). In a standard PCR process, the DNA fragment is combined with starting deoxynucleoside triphosphates, a primer, a buffer and a DNA polymerase in a PCR mixture. The PCR mixture is then reacted in the PCR producing copies of the DNA fragment. The improvement of the present invention is adding an amount of a modifier at any step prior to completion of the PCR process thereby randomly and partially modifying the copies of the DNA fragment asmore » a partially modified PCR product. The partially modified PCR product may then be digested with an enzyme that cuts the partially modified PCR product at unmodified sites thereby producing an array of DNA restriction fragments.« less
  • The present invention is an improved method of making a partially modified PCR product from a DNA fragment with a polymerase chain reaction (PCR). In a standard PCR process, the DNA fragment is combined with starting deoxynucleoside triphosphates, a primer, a buffer and a DNA polymerase in a PCR mixture. The PCR mixture is then reacted in the PCR producing copies of the DNA fragment. The improvement of the present invention is adding an amount of a modifier at any step prior to completion of the PCR process thereby randomly and partially modifying the copies of the DNA fragment asmore » a partially modified PCR product. The partially modified PCR product may then be digested with an enzyme that cuts the partially modified PCR product at unmodified sites thereby producing an array of DNA restriction fragments.« less
  • Waste materials are digested by thermophilic bacteria to produce single-cell protein and vitamin B12. The bacteria are contained in the waste and are not inoculated. Thus, a hog manure slurry containing 10% solids was stirred with aeration in an insulated reactor to allow the temperature to be maintained at greater than 55/sup 0/. The temperature was maintained at 55-65/sup 0/ and the dissolved O/sub 2/ concentration at 1.5-3 ppm for 6 days. After 10 days reaction, the product was fed to hogs as 10% of their nutrient supply with no apparent adverse effects.
  • The present invention generally relates to processes for production of ethanol from cellulosic biomass. The present invention also relates to production of various co-products of preparation of ethanol from cellulosic biomass. The present invention further relates to improvements in one or more aspects of preparation of ethanol from cellulosic biomass including, for example, improved methods for cleaning biomass feedstocks, improved acid impregnation, and improved steam treatment, or "steam explosion."