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Title: Measurement of glutamate in dorsal root ganglion cell culture with integrated electrochemical biosensors

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1357398
Report Number(s):
LLNL-CONF-716997
DOE Contract Number:
AC52-07NA27344
Resource Type:
Conference
Resource Relation:
Conference: Presented at: IEEE Medical Measurements and Applications, Rochester, MN, United States, May 07 - May 10, 2017
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Belle, A M, Enright, H A, Mukerjee, E V, Soscia, D A, Osburn, J J, Kuhn, E A, Kulp, K S, Wheeler, E K, and Tolosa, V M. Measurement of glutamate in dorsal root ganglion cell culture with integrated electrochemical biosensors. United States: N. p., 2017. Web.
Belle, A M, Enright, H A, Mukerjee, E V, Soscia, D A, Osburn, J J, Kuhn, E A, Kulp, K S, Wheeler, E K, & Tolosa, V M. Measurement of glutamate in dorsal root ganglion cell culture with integrated electrochemical biosensors. United States.
Belle, A M, Enright, H A, Mukerjee, E V, Soscia, D A, Osburn, J J, Kuhn, E A, Kulp, K S, Wheeler, E K, and Tolosa, V M. Thu . "Measurement of glutamate in dorsal root ganglion cell culture with integrated electrochemical biosensors". United States. doi:. https://www.osti.gov/servlets/purl/1357398.
@article{osti_1357398,
title = {Measurement of glutamate in dorsal root ganglion cell culture with integrated electrochemical biosensors},
author = {Belle, A M and Enright, H A and Mukerjee, E V and Soscia, D A and Osburn, J J and Kuhn, E A and Kulp, K S and Wheeler, E K and Tolosa, V M},
abstractNote = {},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 05 00:00:00 EST 2017},
month = {Thu Jan 05 00:00:00 EST 2017}
}

Conference:
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  • An autoradiographic method was developed to quantify on a comparative basis the binding of (/sup 3/H)actinomycin D (Act D) to the cell nuclei of frozen, unfixed sections of spinal sensory ganglia in rats. After a crush lesion of the sciatic nerve, alterations of (/sup 3/H)Act D binding were found in L5 and L6 dorsal root ganglia which corresponded to changes in RNA synthesis observed in other studies. An increase in Act D binding was seen at 1 to 3 days postoperation, followed by a decrease at 5 to 7 days. By 9 to 11 days a second increase in bindingmore » occurred, followed by a decrease at 14 days. Contralateral ganglia exhibited an increase in Act D binding only at 5 days compared with unoperated controls. The timing of the response in axotomized ganglia differed with the distance of the lesion from the cell body. The observed patterns of Act D binding confirm that changes of chromatin structure are closely associated with the alterations of RNA and protein synthesis occurring after axon injury. The method may be useful as an indicator for alterations in RNA synthesis related to changes in chromatin structure in complex tissues.« less
  • Thoracic sensory neurons in bullfrog tadpoles can be induced to form connections typical of brachial sensory neurons by transplanting thoracic ganglia to the branchial level at stages when some thoracic sensory neurons already have formed connections. In order to find out how many postmitotic sensory neurons survive transplantation, ({sup 3}H)thymidine was administered to tadpoles in which thoracic ganglia were transplanted to the brachial level unilaterally at stages VII to IX. Between 16 and 37% of the neurons in transplanted ganglia were unlabeled, as compared to 46 to 60% in unoperated ganglia. Transplanted ganglia contained fewer unlabeled neurons than corresponding unoperatedmore » ganglia, indicating that transplantation caused degeneration of postmitotic neurons. Therefore, a large fraction of the neurons that formed connections typical of brachial sensory neurons probably differentiated while they were at the brachial level.« less
  • The expression of major cytoskeletal protein mRNAs was studied in adult rat dorsal root ganglion (DRG) neurons after crushing either their central or peripheral branch axons. mRNA levels in DRG neurons were examined by quantitative in situ hybridization with radiolabeled cDNA probes specific for the low-molecular-weight neurofilament protein (NF-L) and beta-tubulin. The large-sized (greater than 1000 microns 2) neurons which give rise to myelinated axons in lumbar ganglia (L4 and L5) were studied 1 d through 8 weeks after either dorsal root or sciatic nerve crush. NF-L and beta-tubulin mRNA levels in axotomized DRG neurons were compared to those inmore » contralateral control DRG neurons, as well as to those in normal (completely untreated) DRG cells. In the case of NF-L mRNA, changes were observed after central as well as peripheral branch axotomy and the time course and magnitude of changes were similar after both types of axotomy. NF-L mRNA levels initially decreased (first 2 weeks after crush) and then began to return towards control levels at longer survival times. Similar, but less pronounced, changes in NF-L mRNA levels also occurred in contralateral DRG neurons (which were uninjured); the changes in contralateral neurons were not simply a result of surgical stress since no changes in NF-L mRNA levels were observed in sham-operated DRG neurons. In the case of tubulin mRNA, changes were observed after central as well as peripheral branch axotomy by in situ hybridization, but the time course and magnitude of changes were different after each type of axotomy.« less