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Title: Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

Abstract

Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize lignin as a carbon or energy source. Bacillus ligniniphilus L1 was selected in this study due to its capability to utilize alkaline lignin as a single carbon or energy source and its excellent ability to survive in extreme environments. To investigate the aromatic metabolites of strain L1 decomposing alkaline lignin, GC–MS analysis was performed and fifteen single phenol ring aromatic compounds were identified. The dominant absorption peak included phenylacetic acid, 4-hydroxy-benzoicacid, and vanillic acid with the highest proportion of metabolites resulting in 42%. Comparison proteomic analysis was carried out for further study showed that approximately 1447 kinds of proteins were produced, 141 of which were at least twofold up-regulated with alkaline lignin as the single carbon source. The up-regulated proteins contents different categories in the biological functions of protein including lignin degradation, ABC transport system,more » environmental response factors, protein synthesis, assembly, etc. In conclusion, GC–MS analysis showed that alkaline lignin degradation of strain L1 produced 15 kinds of aromatic compounds. Comparison proteomic data and metabolic analysis showed that to ensure the degradation of lignin and growth of strain L1, multiple aspects of cells metabolism including transporter, environmental response factors, and protein synthesis were enhanced. Based on genome and proteomic analysis, at least four kinds of lignin degradation pathway might be present in strain L1, including a Gentisate pathway, the benzoic acid pathway and the β-ketoadipate pathway. The study provides an important basis for lignin degradation by bacteria.« less

Authors:
 [1];  [2];  [2];  [3];  [2];  [4];  [5]
  1. Jiangsu Univ., Zhenjiang, Jiangsu (China). School of Environment and Safety Engineering; Guangdong Inst. of Microbiology, Guangzhou, Guangdong (China). State Key Lab. of Microbial Culture Collection and Application
  2. Jiangsu Univ., Zhenjiang, Jiangsu (China). School of Environment and Safety Engineering
  3. Guangdong Inst. of Microbiology, Guangzhou, Guangdong (China). State Key Lab. of Microbial Culture Collection and Application
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division and Environmental Molecular Sciences Lab.
  5. Washington State Univ., Pullman, WA (United States). Bioproducts, Sciences and Engineering Lab., Dept. of Biological Systems Engineering
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1357047
Report Number(s):
PNNL-SA-124233
Journal ID: ISSN 1754-6834; PII: 735
Grant/Contract Number:  
AC05-76RL01830; EE0006112
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Alkaline lignin; Bacillus ligniniphilus L1; GC–MS; Proteomics

Citation Formats

Zhu, Daochen, Zhang, Peipei, Xie, Changxiao, Zhang, Weimin, Sun, Jianzhong, Qian, Wei-Jun, and Yang, Bin. Biodegradation of alkaline lignin by Bacillus ligniniphilus L1. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0735-y.
Zhu, Daochen, Zhang, Peipei, Xie, Changxiao, Zhang, Weimin, Sun, Jianzhong, Qian, Wei-Jun, & Yang, Bin. Biodegradation of alkaline lignin by Bacillus ligniniphilus L1. United States. doi:10.1186/s13068-017-0735-y.
Zhu, Daochen, Zhang, Peipei, Xie, Changxiao, Zhang, Weimin, Sun, Jianzhong, Qian, Wei-Jun, and Yang, Bin. Tue . "Biodegradation of alkaline lignin by Bacillus ligniniphilus L1". United States. doi:10.1186/s13068-017-0735-y. https://www.osti.gov/servlets/purl/1357047.
@article{osti_1357047,
title = {Biodegradation of alkaline lignin by Bacillus ligniniphilus L1},
author = {Zhu, Daochen and Zhang, Peipei and Xie, Changxiao and Zhang, Weimin and Sun, Jianzhong and Qian, Wei-Jun and Yang, Bin},
abstractNote = {Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize lignin as a carbon or energy source. Bacillus ligniniphilus L1 was selected in this study due to its capability to utilize alkaline lignin as a single carbon or energy source and its excellent ability to survive in extreme environments. To investigate the aromatic metabolites of strain L1 decomposing alkaline lignin, GC–MS analysis was performed and fifteen single phenol ring aromatic compounds were identified. The dominant absorption peak included phenylacetic acid, 4-hydroxy-benzoicacid, and vanillic acid with the highest proportion of metabolites resulting in 42%. Comparison proteomic analysis was carried out for further study showed that approximately 1447 kinds of proteins were produced, 141 of which were at least twofold up-regulated with alkaline lignin as the single carbon source. The up-regulated proteins contents different categories in the biological functions of protein including lignin degradation, ABC transport system, environmental response factors, protein synthesis, assembly, etc. In conclusion, GC–MS analysis showed that alkaline lignin degradation of strain L1 produced 15 kinds of aromatic compounds. Comparison proteomic data and metabolic analysis showed that to ensure the degradation of lignin and growth of strain L1, multiple aspects of cells metabolism including transporter, environmental response factors, and protein synthesis were enhanced. Based on genome and proteomic analysis, at least four kinds of lignin degradation pathway might be present in strain L1, including a Gentisate pathway, the benzoic acid pathway and the β-ketoadipate pathway. The study provides an important basis for lignin degradation by bacteria.},
doi = {10.1186/s13068-017-0735-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {Tue Feb 21 00:00:00 EST 2017},
month = {Tue Feb 21 00:00:00 EST 2017}
}

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Works referenced in this record:

Enzymatic "Combustion": The Microbial Degradation of Lignin
journal, October 1987


The Catalytic Valorization of Lignin for the Production of Renewable Chemicals
journal, June 2010

  • Zakzeski, Joseph; Bruijnincx, Pieter C. A.; Jongerius, Anna L.
  • Chemical Reviews, Vol. 110, Issue 6, p. 3552-3599
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Vanillin oligomerization as a model of side reactions in lignin fragmentation
journal, July 2012