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Title: Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis

Abstract

Eukaryotic cells routinely compartmentalize metabolic pathways to particular organelles for biosynthetic purposes. Relatively few studies have addressed the cellular localization of pathways for secondary metabolites synthesis. In this study, the phytopathogenic fungus Fusarium graminearum reorganized its endoplasmic reticulum (ER) when triggered to produce mycotoxins, both in vitro and in planta. Fluorescence tagged biosynthetic proteins were found to co-localize with the modified ER as confirmed by co-fluorescence and co-purification with known ER proteins. Microscopy, cell sorting, and proteomics were applied in this FICUS collaborative effort.

Authors:
 [1];  [2];  [3];  [3];  [3];  [4];  [4];  [3];  [5]
  1. Univ. of Minnesota, St. Paul, MN (United States)
  2. USDA ARS Cereal Disease Lab., St. Paul, MN (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Oregon State Univ., Corvallis, OR (United States)
  5. USDA ARS Cereal Disease Lab., St. Paul, MN (United States); Univ. of Minnesota, St. Paul, MN (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1356473
Report Number(s):
PNNL-SA-125650
Journal ID: ISSN 2045-2322; KP1704020
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; endoplasmic reticulum; fungi; trichothecenes; fluorescence; proteomics; differentiation; fungal biology

Citation Formats

Boenisch, Marike Johanne, Broz, Karen Lisa, Purvine, Samuel Owen, Chrisler, William Byron, Nicora, Carrie Diana, Connolly, Lanelle Reine, Freitag, Michael, Baker, Scott Edward, and Kistler, Harold Corby. Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis. United States: N. p., 2017. Web. doi:10.1038/srep44296.
Boenisch, Marike Johanne, Broz, Karen Lisa, Purvine, Samuel Owen, Chrisler, William Byron, Nicora, Carrie Diana, Connolly, Lanelle Reine, Freitag, Michael, Baker, Scott Edward, & Kistler, Harold Corby. Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis. United States. doi:10.1038/srep44296.
Boenisch, Marike Johanne, Broz, Karen Lisa, Purvine, Samuel Owen, Chrisler, William Byron, Nicora, Carrie Diana, Connolly, Lanelle Reine, Freitag, Michael, Baker, Scott Edward, and Kistler, Harold Corby. Mon . "Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis". United States. doi:10.1038/srep44296. https://www.osti.gov/servlets/purl/1356473.
@article{osti_1356473,
title = {Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis},
author = {Boenisch, Marike Johanne and Broz, Karen Lisa and Purvine, Samuel Owen and Chrisler, William Byron and Nicora, Carrie Diana and Connolly, Lanelle Reine and Freitag, Michael and Baker, Scott Edward and Kistler, Harold Corby},
abstractNote = {Eukaryotic cells routinely compartmentalize metabolic pathways to particular organelles for biosynthetic purposes. Relatively few studies have addressed the cellular localization of pathways for secondary metabolites synthesis. In this study, the phytopathogenic fungus Fusarium graminearum reorganized its endoplasmic reticulum (ER) when triggered to produce mycotoxins, both in vitro and in planta. Fluorescence tagged biosynthetic proteins were found to co-localize with the modified ER as confirmed by co-fluorescence and co-purification with known ER proteins. Microscopy, cell sorting, and proteomics were applied in this FICUS collaborative effort.},
doi = {10.1038/srep44296},
journal = {Scientific Reports},
number = ,
volume = 7,
place = {United States},
year = {Mon Mar 13 00:00:00 EDT 2017},
month = {Mon Mar 13 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 1 work
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