Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry

Zhou, Mowei; Wu, Si; Stenoien, David L.; Zhang, Zhaorui; Connolly, Lanelle; Freitag, Michael; Pasa-Tolic, Ljiljana

doi:10.1007/978-1-4939-6518-2_12

Title: Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry

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Abstract

Top-down mass spectrometry is a valuable tool for charactering post-translational modifications on histones for understanding of gene control and expression. In this protocol, we describe a top-down workflow using liquid chromatography coupled to mass spectrometry for fast global profiling of changes in histone proteoforms between a wild-type and a mutant of a fungal species. The proteoforms exhibiting different abundances can be subjected to further targeted studies by other mass spectrometric or biochemical assays. This method can be generally adapted for preliminary screening for changes in histone modifications between samples such as wild-type vs. mutant, and control vs. disease.

Authors:: Zhou, Mowei; Wu, Si; Stenoien, David L.; Zhang, Zhaorui; Connolly, Lanelle; Freitag, Michael; Pasa-Tolic, Ljiljana

Publication Date:: Fri Nov 11 00:00:00 EST 2016

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1355115

Report Number(s):: PNNL-SA-113153
47802; KP1704020

DOE Contract Number:: AC05-76RL01830

Resource Type:: Book

Resource Relation:: Related Information: Methods in Molecular Biology: Eukaryotic transcriptional and post-transcriptional gene expression regulation, 1507:153-168

Country of Publication:: United States

Language:: English

Subject:: Environmental Molecular Sciences Laboratory

Citation Formats


                    Zhou, Mowei, Wu, Si, Stenoien, David L., Zhang, Zhaorui, Connolly, Lanelle, Freitag, Michael, and Pasa-Tolic, Ljiljana. Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.  United States: N. p., 2016. 
        Web.  doi:10.1007/978-1-4939-6518-2_12.

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                    Zhou, Mowei, Wu, Si, Stenoien, David L., Zhang, Zhaorui, Connolly, Lanelle, Freitag, Michael, & Pasa-Tolic, Ljiljana. Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.  United States.  https://doi.org/10.1007/978-1-4939-6518-2_12

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                    Zhou, Mowei, Wu, Si, Stenoien, David L., Zhang, Zhaorui, Connolly, Lanelle, Freitag, Michael, and Pasa-Tolic, Ljiljana. 2016.  
        "Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry".  United States.  https://doi.org/10.1007/978-1-4939-6518-2_12.

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@article{osti_1355115,

  title        = {Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry},

  author       = {Zhou, Mowei and Wu, Si and Stenoien, David L. and Zhang, Zhaorui and Connolly, Lanelle and Freitag, Michael and Pasa-Tolic, Ljiljana},

  abstractNote = {Top-down mass spectrometry is a valuable tool for charactering post-translational modifications on histones for understanding of gene control and expression. In this protocol, we describe a top-down workflow using liquid chromatography coupled to mass spectrometry for fast global profiling of changes in histone proteoforms between a wild-type and a mutant of a fungal species. The proteoforms exhibiting different abundances can be subjected to further targeted studies by other mass spectrometric or biochemical assays. This method can be generally adapted for preliminary screening for changes in histone modifications between samples such as wild-type vs. mutant, and control vs. disease.},

  doi          = {10.1007/978-1-4939-6518-2_12},

  url          = {https://www.osti.gov/biblio/1355115},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Fri Nov 11 00:00:00 EST 2016},

  month        = {Fri Nov 11 00:00:00 EST 2016}

}

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