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Title: Biodegradation of alkaline lignin by Bacillus ligniniphilus L1

Abstract

Background: Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize lignin as a carbon or energy source. Bacillus ligniniphilus L1 was selected in this study due to its capability to utilize alkaline lignin as a single carbon or energy source and its excellent ability to survive in extreme environments. Results: To investigate the aromatic metabolites of strain L1 decomposing alkaline lignin, GC-MS analyze was performed and fifteen single phenol ring aromatic compounds were identified. The dominant absorption peak included phenylacetic acid, 4-hydroxy-benzoicacid, and vanillic acid with the highest proportion of metabolites resulting in 42%. Comparison proteomic analysis were carried out for further study showed that approximately 1447 kinds of proteins were produced, 141 of which were at least 2-fold up-regulated with alkaline lignin as the single carbon source. The up-regulated proteins contents different categories in the biological functions of protein including lignin degradation, ABCmore » transport system, environmental response factors, protein synthesis and assembly, etc. Conclusions: GC-MS analysis showed that alkaline lignin degradation of strain L1 produced 15 kinds of aromatic compounds. Comparison proteomic data and metabolic analysis showed that to ensure the degradation of lignin and growth of strain L1, multiple aspects of cells metabolism including transporter, environmental response factors, and protein synthesis were enhanced. Based on genome and proteomic analysis, at least four kinds of lignin degradation pathway might be present in strain L1, including a Gentisate pathway, the benzoic acid pathway and the β-ketoadipate pathway. The study provides an important basis for lignin degradation by bacteria.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1355089
Report Number(s):
PNNL-SA-124233
Journal ID: ISSN 1754-6834; 48247
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Zhu, Daochen, Zhang, Peipei, Xie, Changxiao, Zhang, Weimin, Sun, Jianzhong, Qian, Wei-Jun, and Yang, Bin. Biodegradation of alkaline lignin by Bacillus ligniniphilus L1. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0735-y.
Zhu, Daochen, Zhang, Peipei, Xie, Changxiao, Zhang, Weimin, Sun, Jianzhong, Qian, Wei-Jun, & Yang, Bin. Biodegradation of alkaline lignin by Bacillus ligniniphilus L1. United States. https://doi.org/10.1186/s13068-017-0735-y
Zhu, Daochen, Zhang, Peipei, Xie, Changxiao, Zhang, Weimin, Sun, Jianzhong, Qian, Wei-Jun, and Yang, Bin. Tue . "Biodegradation of alkaline lignin by Bacillus ligniniphilus L1". United States. https://doi.org/10.1186/s13068-017-0735-y.
@article{osti_1355089,
title = {Biodegradation of alkaline lignin by Bacillus ligniniphilus L1},
author = {Zhu, Daochen and Zhang, Peipei and Xie, Changxiao and Zhang, Weimin and Sun, Jianzhong and Qian, Wei-Jun and Yang, Bin},
abstractNote = {Background: Lignin is the most abundant aromatic biopolymer in the biosphere and it comprises up to 30% of plant biomass. Although lignin is the most recalcitrant component of the plant cell wall, still there are microorganisms able to decompose it or degrade it. Fungi are recognized as the most widely used microbes for lignin degradation. However, bacteria have also been known to be able to utilize lignin as a carbon or energy source. Bacillus ligniniphilus L1 was selected in this study due to its capability to utilize alkaline lignin as a single carbon or energy source and its excellent ability to survive in extreme environments. Results: To investigate the aromatic metabolites of strain L1 decomposing alkaline lignin, GC-MS analyze was performed and fifteen single phenol ring aromatic compounds were identified. The dominant absorption peak included phenylacetic acid, 4-hydroxy-benzoicacid, and vanillic acid with the highest proportion of metabolites resulting in 42%. Comparison proteomic analysis were carried out for further study showed that approximately 1447 kinds of proteins were produced, 141 of which were at least 2-fold up-regulated with alkaline lignin as the single carbon source. The up-regulated proteins contents different categories in the biological functions of protein including lignin degradation, ABC transport system, environmental response factors, protein synthesis and assembly, etc. Conclusions: GC-MS analysis showed that alkaline lignin degradation of strain L1 produced 15 kinds of aromatic compounds. Comparison proteomic data and metabolic analysis showed that to ensure the degradation of lignin and growth of strain L1, multiple aspects of cells metabolism including transporter, environmental response factors, and protein synthesis were enhanced. Based on genome and proteomic analysis, at least four kinds of lignin degradation pathway might be present in strain L1, including a Gentisate pathway, the benzoic acid pathway and the β-ketoadipate pathway. The study provides an important basis for lignin degradation by bacteria.},
doi = {10.1186/s13068-017-0735-y},
url = {https://www.osti.gov/biblio/1355089}, journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {2}
}

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