skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound

Abstract

The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.

Inventors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1354809
Patent Number(s):
9,637,766
Application Number:
15/186,202
Assignee:
The Regents of the University of California LBNL
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Jun 17
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Kirby, James, Fortman, Jeffrey L., Nishimoto, Minobu, and Keasling, Jay D. Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound. United States: N. p., 2017. Web.
Kirby, James, Fortman, Jeffrey L., Nishimoto, Minobu, & Keasling, Jay D. Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound. United States.
Kirby, James, Fortman, Jeffrey L., Nishimoto, Minobu, and Keasling, Jay D. Tue . "Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound". United States. doi:. https://www.osti.gov/servlets/purl/1354809.
@article{osti_1354809,
title = {Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound},
author = {Kirby, James and Fortman, Jeffrey L. and Nishimoto, Minobu and Keasling, Jay D.},
abstractNote = {The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 02 00:00:00 EDT 2017},
month = {Tue May 02 00:00:00 EDT 2017}
}

Patent:

Save / Share:

Works referenced in this record:

Biosynthesis of isoprenoids via the non-mevalonate pathway
journal, June 2004

  • Eisenreich, W.; Bacher, A.; Arigoni, D.
  • Cellular and Molecular Life Sciences, Vol. 61, Issue 12, p. 1401-1426
  • DOI: 10.1007/s00018-004-3381-z

Computational Analysis of the Evolution of 1-Deoxy-D-xylulose-5-phosphate Reductoisomerase, an Important Enzyme in Plant Terpene Biosynthesis
journal, May 2010

  • Fung, Pui Kwan; Krushkal, Julia; Weathers, Pamela J.
  • Chemistry & Biodiversity, Vol. 7, Issue 5, p. 1098-1110
  • DOI: 10.1002/cbdv.200900313

Phosphorylation of 1-deoxy-D-xylulose by D-xylulokinase of Escherichia coli:
journal, January 2001


Conversion of Methylglyoxal to Acetol by Escherichia coli Aldo-Keto Reductases
journal, August 2005


Mutations in Escherichia coli aceE and ribB Genes Allow Survival of Strains Defective in the First Step of the Isoprenoid Biosynthesis Pathway
journal, August 2012


Mono and diterpene production in Escherichia coli
journal, January 2004

  • Reiling, K. Kinkead; Yoshikuni, Yasuo; Martin, Vincent J.J.
  • Biotechnology and Bioengineering, Vol. 87, Issue 2, p. 200-212
  • DOI: 10.1002/bit.20128

A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy- d -xylulose 5-phosphate synthase
journal, January 2006


Identification of Isopentenol Biosynthetic Genes from Bacillus subtilis by a Screening Method Based on Isoprenoid Precursor Toxicity
journal, August 2007

  • Withers, S. T.; Gottlieb, S. S.; Lieu, B.
  • Applied and Environmental Microbiology, Vol. 73, Issue 19, p. 6277-6283
  • DOI: 10.1128/AEM.00861-07