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Title: Fluorescence and confocal imaging of mammalian cells using conjugated oligoelectrolytes with phenylenevinylene core

Abstract

Over the last few years, considerable efforts are taken, in order to find a molecular fluorescent probe fulfilling their applicability requirements. Due to a good optical properties and affinity to biological structures conjugated oligoelectrolytes (COEs) can be considered as a promising dyes for application in fluorescence-based bioimaging. In this work, we synthetized COEs with phenylenevinylene core (PV-COEs) and applied as fluorescent membranous-specific probes. Cytotoxicity effects of each COE were probed on cancerous and non-cancerous cell types and little to no toxicity effects were observed at the high range of concentrations. The intensity of cell fluorescence following the COE staining was determined by the photoluminescence analysis and fluorescence activated cell sorting method (FACS). Intercalation of tested COEs into mammalian cell membranes was revealed by fluorescent and confocal microscopy colocalization with commercial dyes specific for cellular structures including mitochondria, Golgi apparatus and endoplasmic reticulum. The phenylenevinylene conjugated oligoelectrolytes have been found to be suitable for fluorescent bioimaging of mammalian cells and membrane-rich organelles. Due to their water solubility coupled with spontaneous intercalation into cells, favorable photophysical features, ease of cell staining, low cytotoxicity and selectivity for membranous structures, PV-COEs can be applied as markers for fluorescence imaging of a variety of cellmore » types.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1353001
Report Number(s):
NREL/JA-5900-68397
Journal ID: ISSN 1011-1344
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Photochemistry and Photobiology B: Biology; Journal Volume: 170; Journal Issue: C
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; conjugated oligoelectrolytes; membrane stain; fluorescence imaging; cytotoxicity; cell imaging

Citation Formats

Milczarek, Justyna, Pawlowska, Roza, Zurawinski, Remigiusz, Lukasik, Beata, Garner, Logan E., and Chworos, Arkadiusz. Fluorescence and confocal imaging of mammalian cells using conjugated oligoelectrolytes with phenylenevinylene core. United States: N. p., 2017. Web. doi:10.1016/j.jphotobiol.2017.03.017.
Milczarek, Justyna, Pawlowska, Roza, Zurawinski, Remigiusz, Lukasik, Beata, Garner, Logan E., & Chworos, Arkadiusz. Fluorescence and confocal imaging of mammalian cells using conjugated oligoelectrolytes with phenylenevinylene core. United States. doi:10.1016/j.jphotobiol.2017.03.017.
Milczarek, Justyna, Pawlowska, Roza, Zurawinski, Remigiusz, Lukasik, Beata, Garner, Logan E., and Chworos, Arkadiusz. Mon . "Fluorescence and confocal imaging of mammalian cells using conjugated oligoelectrolytes with phenylenevinylene core". United States. doi:10.1016/j.jphotobiol.2017.03.017.
@article{osti_1353001,
title = {Fluorescence and confocal imaging of mammalian cells using conjugated oligoelectrolytes with phenylenevinylene core},
author = {Milczarek, Justyna and Pawlowska, Roza and Zurawinski, Remigiusz and Lukasik, Beata and Garner, Logan E. and Chworos, Arkadiusz},
abstractNote = {Over the last few years, considerable efforts are taken, in order to find a molecular fluorescent probe fulfilling their applicability requirements. Due to a good optical properties and affinity to biological structures conjugated oligoelectrolytes (COEs) can be considered as a promising dyes for application in fluorescence-based bioimaging. In this work, we synthetized COEs with phenylenevinylene core (PV-COEs) and applied as fluorescent membranous-specific probes. Cytotoxicity effects of each COE were probed on cancerous and non-cancerous cell types and little to no toxicity effects were observed at the high range of concentrations. The intensity of cell fluorescence following the COE staining was determined by the photoluminescence analysis and fluorescence activated cell sorting method (FACS). Intercalation of tested COEs into mammalian cell membranes was revealed by fluorescent and confocal microscopy colocalization with commercial dyes specific for cellular structures including mitochondria, Golgi apparatus and endoplasmic reticulum. The phenylenevinylene conjugated oligoelectrolytes have been found to be suitable for fluorescent bioimaging of mammalian cells and membrane-rich organelles. Due to their water solubility coupled with spontaneous intercalation into cells, favorable photophysical features, ease of cell staining, low cytotoxicity and selectivity for membranous structures, PV-COEs can be applied as markers for fluorescence imaging of a variety of cell types.},
doi = {10.1016/j.jphotobiol.2017.03.017},
journal = {Journal of Photochemistry and Photobiology B: Biology},
number = C,
volume = 170,
place = {United States},
year = {Mon May 01 00:00:00 EDT 2017},
month = {Mon May 01 00:00:00 EDT 2017}
}