skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

Abstract

The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.

Inventors:
;
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1351831
Patent Number(s):
9,625,387
Application Number:
14/487,997
Assignee:
Lawrence Livermore National Security, LLC LLNL
DOE Contract Number:
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Sep 16
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 36 MATERIALS SCIENCE

Citation Formats

Demos, Stavros, and Levenson, Richard. System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents. United States: N. p., 2017. Web.
Demos, Stavros, & Levenson, Richard. System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents. United States.
Demos, Stavros, and Levenson, Richard. Tue . "System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents". United States. doi:. https://www.osti.gov/servlets/purl/1351831.
@article{osti_1351831,
title = {System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents},
author = {Demos, Stavros and Levenson, Richard},
abstractNote = {The present disclosure relates to a method for analyzing tissue specimens. In one implementation the method involves obtaining a tissue sample and exposing the sample to one or more fluorophores as contrast agents to enhance contrast of subcellular compartments of the tissue sample. The tissue sample is illuminated by an ultraviolet (UV) light having a wavelength between about 200 nm to about 400 nm, with the wavelength being selected to result in penetration to only a specified depth below a surface of the tissue sample. Inter-image operations between images acquired under different imaging parameters allow for improvement of the image quality via removal of unwanted image components. A microscope may be used to image the tissue sample and provide the image to an image acquisition system that makes use of a camera. The image acquisition system may create a corresponding image that is transmitted to a display system for processing and display.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 18 00:00:00 EDT 2017},
month = {Tue Apr 18 00:00:00 EDT 2017}
}

Patent:

Save / Share:
  • This patent describes a spectroscopic scanning tunneling microscope (SSTM) apparatus for differential atomic imaging the surface of a material sample. It comprises: a mounting stage for mounting the sample; a fine-point electrode probe positioned adjacent the mounting stage and being positionable very closely adjacent a sample that is mounted on the stage to accommodate a tunneling current between the sample and the probe; tunable electronic surface bias means connected to the surface of the sample and to the probe for electronically biasing the surface of the sample in relation to the probe with a surface voltage bias; photon bias meansmore » adjacent the stage for providing photon biasing of selected wavelengths and frequencies on the surface of the sample adjacent the probe; instrumentation means for measuring tunneling current through the probe and electronic voltage bias; data processing means; and display means.« less
  • A Method and apparatus for differential spectroscopic atomic-imaging is disclosed for spatial resolution and imaging for display not only individual atoms on a sample surface, but also bonding and the specific atomic species in such bond. The apparatus includes a scanning tunneling microscope (STM) that is modified to include photon biasing, preferably a tuneable laser, modulating electronic surface biasing for the sample, and temperature biasing, preferably a vibration-free refrigerated sample mounting stage. Computer control and data processing and visual display components are also included. The method includes modulating the electronic bias voltage with and without selected photon wavelengths and frequencymore » biasing under a stabilizing (usually cold) bias temperature to detect bonding and specific atomic species in the bonds as the STM rasters the sample. This data is processed along with atomic spatial topography data obtained from the STM raster scan to create a real-time visual image of the atoms on the sample surface.« less
  • A microscopy imaging system includes a first light source for providing a first train of pulses at a first center optical frequency .omega..sub.1, a second light source for providing a second train of pulses at a second center optical frequency .omega..sub.2, a modulator system, an optical detector, and a processor. The modulator system is for modulating a beam property of the second train of pulses at a modulation frequency f of at least 100 kHz. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of the first train of pulses from the common focalmore » volume by blocking the second train of pulses being modulated. The processor is for detecting, a modulation at the modulation frequency f, of the integrated intensity of the optical frequency components of the first train of pulses to provide a pixel of an image for the microscopy imaging system.« less
  • In an imaging NMR scanner of the type which includes means which repetitively tests selected internal regions of an object by transmitting an initial NMR r.f. nutation pulse followed by at least one 180/sup 0/ NMR r.f. nutation pulse so as to elicit at least one NMR spin echo signal from a selected region during each of successive NMR measurement cycles, this patent describes an improvement comprising: control means for causing the initial NMR r.f. nutation pulse to produce a first nutation angle phi/sub 1/ during at least one first NMR measurement cycle and to produce a different second nutationmore » angle phi/sub 2/ during at least one other second NMR measurement cycle.« less
  • Selective excitation is used to define a thick planar slab of excited nuclear spins in a nuclear magnetic resonance (NMR) imaging sample. The thick slab is selected such that the excited spins are contained well within the optimum sensitive region defined by the radio frequency (RF) transmitter and receiver coils. Three-dimensional spatial information of an NMR imaging parameter, such as nuclear spin density or nuclear spin relaxation time, is collected simultaneously from the excited slab and can be used to construct a series of several tomographic section images of the slab. The spatial information is encoded in the NMR signalmore » by application of pulsed gradient magnetic fields subsequent to excitation. Image picture information is obtained from the NMR signals via three-dimensional Fourier transformation.« less