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Title: Gene expression analysis by arylsulfatase assays in the green alga Chlamydomonas reinhardtii

Abstract

Chlamydomonas reinhardtii, a single-celled green alga, is a powerful microbial experimental system for understanding gene function. As a consequence of a high-quality genome sequence, community-wide efforts for gene model refinement and annotation, resources for strain collections and robust molecular techniques, research with this organism has significantly expanded in the past few decades. In two companion articles, we outline colorimetric and fluorescence-based methodologies for genetic reporter systems in Chlamydomonas, which can be used to investigate and delineate gene expression and regulatory mechanisms. As a result, we describe protocols for arylsulfatase activity assays using ARS2, activity of which can be measured either quantitatively or qualitatively, and in low (individual sample) or high (96-well format) throughput.

Authors:
 [1];  [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1351730
Report Number(s):
BNL-113718-2017-JA
Journal ID: ISSN 1064-3745
Grant/Contract Number:  
SC0012704
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Methods in Molecular Biology
Additional Journal Information:
Journal Volume: 1755; Journal ID: ISSN 1064-3745
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Algae; ARS2; gene fusion; reporter; high-throughput assay

Citation Formats

Blaby, Ian K., and Blaby-Haas, Crysten E. Gene expression analysis by arylsulfatase assays in the green alga Chlamydomonas reinhardtii. United States: N. p., 2018. Web. doi:10.1007/978-1-4939-7724-6_11.
Blaby, Ian K., & Blaby-Haas, Crysten E. Gene expression analysis by arylsulfatase assays in the green alga Chlamydomonas reinhardtii. United States. doi:10.1007/978-1-4939-7724-6_11.
Blaby, Ian K., and Blaby-Haas, Crysten E. Thu . "Gene expression analysis by arylsulfatase assays in the green alga Chlamydomonas reinhardtii". United States. doi:10.1007/978-1-4939-7724-6_11. https://www.osti.gov/servlets/purl/1351730.
@article{osti_1351730,
title = {Gene expression analysis by arylsulfatase assays in the green alga Chlamydomonas reinhardtii},
author = {Blaby, Ian K. and Blaby-Haas, Crysten E.},
abstractNote = {Chlamydomonas reinhardtii, a single-celled green alga, is a powerful microbial experimental system for understanding gene function. As a consequence of a high-quality genome sequence, community-wide efforts for gene model refinement and annotation, resources for strain collections and robust molecular techniques, research with this organism has significantly expanded in the past few decades. In two companion articles, we outline colorimetric and fluorescence-based methodologies for genetic reporter systems in Chlamydomonas, which can be used to investigate and delineate gene expression and regulatory mechanisms. As a result, we describe protocols for arylsulfatase activity assays using ARS2, activity of which can be measured either quantitatively or qualitatively, and in low (individual sample) or high (96-well format) throughput.},
doi = {10.1007/978-1-4939-7724-6_11},
journal = {Methods in Molecular Biology},
issn = {1064-3745},
number = ,
volume = 1755,
place = {United States},
year = {2018},
month = {4}
}

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Works referenced in this record:

The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions
journal, October 2007

  • Merchant, S. S.; Prochnik, S. E.; Vallon, O.
  • Science, Vol. 318, Issue 5848, p. 245-250
  • DOI: 10.1126/science.1143609