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Title: Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis

Abstract

Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Here we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1351394
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Virology; Journal Volume: 89; Journal Issue: 9
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., Basler, Christopher F., and Lyles, D. S.. Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis. United States: N. p., 2015. Web. doi:10.1128/JVI.03652-14.
Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., Basler, Christopher F., & Lyles, D. S.. Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis. United States. doi:10.1128/JVI.03652-14.
Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., Basler, Christopher F., and Lyles, D. S.. Wed . "Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis". United States. doi:10.1128/JVI.03652-14.
@article{osti_1351394,
title = {Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis},
author = {Luthra, Priya and Jordan, David S. and Leung, Daisy W. and Amarasinghe, Gaya K. and Basler, Christopher F. and Lyles, D. S.},
abstractNote = {Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Here we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.},
doi = {10.1128/JVI.03652-14},
journal = {Journal of Virology},
number = 9,
volume = 89,
place = {United States},
year = {Wed Mar 04 00:00:00 EST 2015},
month = {Wed Mar 04 00:00:00 EST 2015}
}