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Title: Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements

Abstract

Diversity-generating retroelements (DGRs) provide organisms with a unique means for adaptation to a dynamic environment through massive protein sequence variation. The potential scope of this variation exceeds that of the vertebrate adaptive immune system. DGRs were known to exist only in viruses and bacteria until their recent discovery in archaea belonging to the ‘microbial dark matter’, specifically in organisms closely related to Nanoarchaeota. However, Nanoarchaeota DGR variable proteins were unassignable to known protein folds and apparently unrelated to characterized DGR variable proteins. To address the issue of how Nanoarchaeota DGR variable proteins accommodate massive sequence variation, we determined the 2.52 Å resolution limit crystal structure of one such protein, AvpA, which revealed a C-type lectin (CLec)-fold that organizes a putative ligand-binding site that is capable of accommodating 1013 sequences. This fold is surprisingly reminiscent of the CLec-folds of viral and bacterial DGR variable protein, but differs sufficiently to define a new CLec-fold subclass, which is consistent with early divergence between bacterial and archaeal DGRs. The structure also enabled identification of a group of AvpA-like proteins in multiple putative DGRs from uncultivated archaea. These variable proteins may aid Nanoarchaeota and these uncultivated archaea in symbiotic relationships. Our results have uncovered themore » widespread conservation of the CLec-fold in viruses, bacteria, and archaea for accommodating massive sequence variation. In addition, to our knowledge, this is the first report of an archaeal CLec-fold protein.« less

Authors:
 [1];  [2];  [3];  [2]; ORCiD logo [1]
  1. Univ. of California, San Diego, La Jolla, CA (United States)
  2. Univ. of California, Santa Barbara, CA (United States)
  3. Univ. of California, Los Angeles, CA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1351348
Grant/Contract Number:  
R01 AI096838; OCE-1046144
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BMC Structural Biology (Online)
Additional Journal Information:
Journal Volume: 16; Journal Issue: 1; Journal ID: ISSN 1472-6807
Publisher:
BioMed Central
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Handa, Sumit, Paul, Blair G., Miller, Jeffery F., Valentine, David L., and Ghosh, Partho. Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements. United States: N. p., 2016. Web. doi:10.1186/s12900-016-0064-6.
Handa, Sumit, Paul, Blair G., Miller, Jeffery F., Valentine, David L., & Ghosh, Partho. Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements. United States. doi:10.1186/s12900-016-0064-6.
Handa, Sumit, Paul, Blair G., Miller, Jeffery F., Valentine, David L., and Ghosh, Partho. Wed . "Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements". United States. doi:10.1186/s12900-016-0064-6. https://www.osti.gov/servlets/purl/1351348.
@article{osti_1351348,
title = {Conservation of the C-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements},
author = {Handa, Sumit and Paul, Blair G. and Miller, Jeffery F. and Valentine, David L. and Ghosh, Partho},
abstractNote = {Diversity-generating retroelements (DGRs) provide organisms with a unique means for adaptation to a dynamic environment through massive protein sequence variation. The potential scope of this variation exceeds that of the vertebrate adaptive immune system. DGRs were known to exist only in viruses and bacteria until their recent discovery in archaea belonging to the ‘microbial dark matter’, specifically in organisms closely related to Nanoarchaeota. However, Nanoarchaeota DGR variable proteins were unassignable to known protein folds and apparently unrelated to characterized DGR variable proteins. To address the issue of how Nanoarchaeota DGR variable proteins accommodate massive sequence variation, we determined the 2.52 Å resolution limit crystal structure of one such protein, AvpA, which revealed a C-type lectin (CLec)-fold that organizes a putative ligand-binding site that is capable of accommodating 1013 sequences. This fold is surprisingly reminiscent of the CLec-folds of viral and bacterial DGR variable protein, but differs sufficiently to define a new CLec-fold subclass, which is consistent with early divergence between bacterial and archaeal DGRs. The structure also enabled identification of a group of AvpA-like proteins in multiple putative DGRs from uncultivated archaea. These variable proteins may aid Nanoarchaeota and these uncultivated archaea in symbiotic relationships. Our results have uncovered the widespread conservation of the CLec-fold in viruses, bacteria, and archaea for accommodating massive sequence variation. In addition, to our knowledge, this is the first report of an archaeal CLec-fold protein.},
doi = {10.1186/s12900-016-0064-6},
journal = {BMC Structural Biology (Online)},
issn = {1472-6807},
number = 1,
volume = 16,
place = {United States},
year = {2016},
month = {8}
}

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