Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells

Lipus, Daniel; Vikram, Amit; Ross, Daniel; Bain, Daniel; Gulliver, Djuna; Hammack, Richard; Bibby, Kyle

doi:10.1128/AEM.02659-16

Title: Predominance and metabolic potential of Halanaerobium spp. in produced water from hydraulically fractured Marcellus Shale wells

Journal Article · Fri Feb 03 00:00:00 EST 2017 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.02659-16· OSTI ID:1351183

Lipus, Daniel ^[1]; Vikram, Amit ^[2]; Ross, Daniel ^[3]; Bain, Daniel ^[2]; Gulliver, Djuna ^[4]; Hammack, Richard ^[4];

^[2]

National Energy Technology Lab. (NETL), Pittsburgh, PA (United States); Univ. of Pittsburgh, Pittsburgh, PA (United States)
Univ. of Pittsburgh, Pittsburgh, PA (United States)
National Energy Technology Lab. (NETL), Pittsburgh, PA (United States); AECOM, Pittsburgh, PA (United States)
National Energy Technology Lab. (NETL), Pittsburgh, PA (United States)

Here, microbial activity in the produced water from hydraulically fractured oil and gas wells may potentially interfere with hydrocarbon production and cause damage to the well and surface infrastructure via corrosion, sulfide release, and fouling. In this study, we surveyed the microbial abundance and community structure of produced water sampled from 42 Marcellus Shale wells in southwestern Pennsylvania (well age ranged from 150 to 1,846 days) to better understand the microbial diversity of produced water. We sequenced the V4 region of the 16S rRNA gene to assess taxonomy and utilized quantitative PCR (qPCR) to evaluate the microbial abundance across all 42 produced water samples. Bacteria of the order Halanaerobiales were found to be the most abundant organisms in the majority of the produced water samples, emphasizing their previously suggested role in hydraulic fracturing-related microbial activity. Statistical analyses identified correlations between well age and biocide formulation and the microbial community, in particular, the relative abundance of Halanaerobiales. We further investigated the role of members of the order Halanaerobiales in produced water by reconstructing and annotating a Halanaerobium draft genome (named MDAL1), using shotgun metagenomic sequencing and metagenomic binning. The recovered draft genome was found to be closely related to the species H. congolense, an oil field isolate, and Halanaerobium sp. strain T82-1, also recovered from hydraulic fracturing produced water. Reconstruction of metabolic pathways revealed Halanaerobium sp. strain MDAL1 to have the potential for acid production, thiosulfate reduction, and biofilm formation, suggesting it to have the ability to contribute to corrosion, souring, and biofouling events in the hydraulic fracturing infrastructure.

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Research Organization:: National Energy Technology Lab. (NETL), Pittsburgh, PA, and Morgantown, WV (United States). In-house Research

Sponsoring Organization:: USDOE

Grant/Contract Number:: RES1000027/183U

OSTI ID:: 1351183

Report Number(s):: NETL-PUB-20135

Journal Information:: Applied and Environmental Microbiology, Vol. 83, Issue 8; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 48 works

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