The sequence context-dependent mispairing of 5-hydroxycytosine and 5-hydroxyuridine in vitro
- Univ. of Vermont, Burlington, VT (United States); and others
Two major stable oxidation products of 2{prime}-deoxycytidine are 2{prime}-deoxy-5-hydroxycytidine (5-OHdC) and 2{prime}-deoxy-5-hydroxyuridine (5-OHdU). Because the 5-hydroxypyrimidines are readily formed on DNA by active oxygen species, it is important to determine their mutagenic potential. In order to study the incorporation of 5-OHdC and 5-OHdU into DNA by DNA polymerase in vitro and to check their base-pairing abilities, we synthesized both 5-OHdCTP and 5-OHdUTP. The specificity of nucleotide incorporation opposite 5-OHdC and 5-OHdU was found to be sequence-context dependent. These data suggest that the 5-hydroxypyrimidines studied have the potential to be mutagenic during incorporation into DNA and translesion DNA synthesis.
- Research Organization:
- New York Academy of Sciences, New York, NY (United States)
- OSTI ID:
- 134878
- Report Number(s):
- CONF-9307221-; TRN: 95:007741-0045
- Resource Relation:
- Conference: DNA damage: effects on DNA structure and protein recognition, Burlington, VT (United States), 31 Jul - 4 Aug 1993; Other Information: PBD: 1994; Related Information: Is Part Of DNA damage: Effects on DNA structure and protein recognition; Wallace, S.S.; Van Houten, B.; Kow, Yoke Wah [eds.]; PB: 395 p.
- Country of Publication:
- United States
- Language:
- English
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