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Title: Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana

Abstract

The plant hormone ethylene is perceived by a five-member family of receptors in Arabidopsis thaliana. The receptors function in conjunction with the Raf-like kinase CTR1 to negatively regulate ethylene signal transduction. CTR1 interacts with multiple members of the receptor family based on co-purification analysis, interacting more strongly with receptors containing a receiver domain. Levels of membrane-associated CTR1 vary in response to ethylene, doing so in a post-transcriptional manner that correlates with ethylene-mediated changes in levels of the ethylene receptors ERS1, ERS2, EIN4, and ETR2. Interactions between CTR1 and the receptor ETR1 protect ETR1 from ethylene-induced turnover. Kinetic and dose-response analyses support a model in which two opposing factors control levels of the ethylene receptor/CTR1 complexes. Ethylene stimulates the production of new complexes largely through transcriptional induction of the receptors. However, ethylene also induces turnover of receptors, such that levels of ethylene receptor/CTR1 complexes decrease at higher ethylene concentrations. Lastly, we discuss implications of this model for ethylene signaling.

Authors:
 [1];  [2];  [1];  [3];  [1];  [1];  [2]
  1. Dartmouth College, Hanover, NH (United States). Dept. of Biological Sciences; Quaid-i-azam Univ., Islamabad (Pakistan). Dept. of Biochemistry
  2. Dartmouth College, Hanover, NH (United States). Dept. of Biological Sciences
  3. Dartmouth College, Hanover, NH (United States). Dept. of Biological Sciences; Jiangsu Academy of Agricultural Sciences, Nanjing (China). Inst. of Biotechnology
Publication Date:
Research Org.:
Dartmouth College, Hanover, NH (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); Higher Education Commission of Pakistan
Contributing Org.:
Quaid-i-azam Univ., Islamabad (Pakistan); Jiangsu Academy of Agricultural Sciences, Nanjing (China)
OSTI Identifier:
1348379
Grant/Contract Number:
FG02-05ER15704
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 290; Journal Issue: 19; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Arabidopsis; ethylene; histidine kinase; hormone receptor; membrane protein; protein complex; protein turnover; serine/threonine protein kinase

Citation Formats

Shakeel, Samina N., Gao, Zhiyong, Amir, Madiha, Chen, Yi-Feng, Rai, Muneeza Iqbal, Haq, Noor Ul, and Schaller, George Eric. Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana. United States: N. p., 2015. Web. doi:10.1074/jbc.m115.652503.
Shakeel, Samina N., Gao, Zhiyong, Amir, Madiha, Chen, Yi-Feng, Rai, Muneeza Iqbal, Haq, Noor Ul, & Schaller, George Eric. Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana. United States. doi:10.1074/jbc.m115.652503.
Shakeel, Samina N., Gao, Zhiyong, Amir, Madiha, Chen, Yi-Feng, Rai, Muneeza Iqbal, Haq, Noor Ul, and Schaller, George Eric. Thu . "Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana". United States. doi:10.1074/jbc.m115.652503. https://www.osti.gov/servlets/purl/1348379.
@article{osti_1348379,
title = {Ethylene Regulates Levels of Ethylene Receptor/CTR1 Signaling Complexes in Arabidopsis thaliana},
author = {Shakeel, Samina N. and Gao, Zhiyong and Amir, Madiha and Chen, Yi-Feng and Rai, Muneeza Iqbal and Haq, Noor Ul and Schaller, George Eric},
abstractNote = {The plant hormone ethylene is perceived by a five-member family of receptors in Arabidopsis thaliana. The receptors function in conjunction with the Raf-like kinase CTR1 to negatively regulate ethylene signal transduction. CTR1 interacts with multiple members of the receptor family based on co-purification analysis, interacting more strongly with receptors containing a receiver domain. Levels of membrane-associated CTR1 vary in response to ethylene, doing so in a post-transcriptional manner that correlates with ethylene-mediated changes in levels of the ethylene receptors ERS1, ERS2, EIN4, and ETR2. Interactions between CTR1 and the receptor ETR1 protect ETR1 from ethylene-induced turnover. Kinetic and dose-response analyses support a model in which two opposing factors control levels of the ethylene receptor/CTR1 complexes. Ethylene stimulates the production of new complexes largely through transcriptional induction of the receptors. However, ethylene also induces turnover of receptors, such that levels of ethylene receptor/CTR1 complexes decrease at higher ethylene concentrations. Lastly, we discuss implications of this model for ethylene signaling.},
doi = {10.1074/jbc.m115.652503},
journal = {Journal of Biological Chemistry},
number = 19,
volume = 290,
place = {United States},
year = {Thu Mar 26 00:00:00 EDT 2015},
month = {Thu Mar 26 00:00:00 EDT 2015}
}

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