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Title: Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae

Abstract

Coenzyme Q (Q or ubiquinone) is a redox active lipid composed of a fully substituted benzoquinone ring and a polyisoprenoid tail and is required for mitochondrial electron transport. In the yeast Saccharomyces cerevisiae, Q is synthesized by the products of 11 known genes, COQ1–COQ9, YAH1, and ARH1. The function of some of the Coq proteins remains unknown, and several steps in the Q biosynthetic pathway are not fully characterized. Several of the Coq proteins are associated in a macromolecular complex on the matrix face of the inner mitochondrial membrane, and this complex is required for efficient Q synthesis. In this paper, we further characterize this complex via immunoblotting and proteomic analysis of tandem affinity-purified tagged Coq proteins. We show that Coq8, a putative kinase required for the stability of the Q biosynthetic complex, is associated with a Coq6-containing complex. Additionally Q 6 and late stage Q biosynthetic intermediates were also found to co-purify with the complex. A mitochondrial protein of unknown function, encoded by the YLR290C open reading frame, is also identified as a constituent of the complex and is shown to be required for efficient de novo Q biosynthesis. Finally, given its effect on Q synthesis and its associationmore » with the biosynthetic complex, we propose that the open reading frame YLR290C be designated COQ11.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [1]
  1. Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. Molecular Biology Inst.
  2. Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. Molecular Biology Inst. UCLA/DOE Inst. for Genomics and Proteomics
  3. Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. Molecular Biology Inst. UCLA/DOE Inst. for Genomics and Proteomics. Dept. of Biological Chemistry
Publication Date:
Research Org.:
Univ. of California, Los Angeles, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Inst. of Health (NIH) (United States); National Science Foundation (NSF)
OSTI Identifier:
1348353
Grant/Contract Number:
FC02-02ER63421; S10RR024605; R01GM103479; MCB-1330803; GM100753
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 290; Journal Issue: 12; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Coenzyme Q; Immunoprecipitation; Mass Spectrometry (MS); Mitochondrial Metabolism; Protein Complex; Proteomics; Q Biosynthetic Intermediates; Saccharomyces cerevisiae; Ubiquinone; Yeast

Citation Formats

Allan, Christopher M., Awad, Agape M., Johnson, Jarrett S., Shirasaki, Dyna I., Wang, Charles, Blaby-Haas, Crysten E., Merchant, Sabeeha S., Loo, Joseph A., and Clarke, Catherine F. Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae. United States: N. p., 2015. Web. doi:10.1074/jbc.m114.633131.
Allan, Christopher M., Awad, Agape M., Johnson, Jarrett S., Shirasaki, Dyna I., Wang, Charles, Blaby-Haas, Crysten E., Merchant, Sabeeha S., Loo, Joseph A., & Clarke, Catherine F. Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae. United States. doi:10.1074/jbc.m114.633131.
Allan, Christopher M., Awad, Agape M., Johnson, Jarrett S., Shirasaki, Dyna I., Wang, Charles, Blaby-Haas, Crysten E., Merchant, Sabeeha S., Loo, Joseph A., and Clarke, Catherine F. Wed . "Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae". United States. doi:10.1074/jbc.m114.633131. https://www.osti.gov/servlets/purl/1348353.
@article{osti_1348353,
title = {Identification of Coq11, a New Coenzyme Q Biosynthetic Protein in the CoQ-Synthome in Saccharomyces cerevisiae},
author = {Allan, Christopher M. and Awad, Agape M. and Johnson, Jarrett S. and Shirasaki, Dyna I. and Wang, Charles and Blaby-Haas, Crysten E. and Merchant, Sabeeha S. and Loo, Joseph A. and Clarke, Catherine F.},
abstractNote = {Coenzyme Q (Q or ubiquinone) is a redox active lipid composed of a fully substituted benzoquinone ring and a polyisoprenoid tail and is required for mitochondrial electron transport. In the yeast Saccharomyces cerevisiae, Q is synthesized by the products of 11 known genes, COQ1–COQ9, YAH1, and ARH1. The function of some of the Coq proteins remains unknown, and several steps in the Q biosynthetic pathway are not fully characterized. Several of the Coq proteins are associated in a macromolecular complex on the matrix face of the inner mitochondrial membrane, and this complex is required for efficient Q synthesis. In this paper, we further characterize this complex via immunoblotting and proteomic analysis of tandem affinity-purified tagged Coq proteins. We show that Coq8, a putative kinase required for the stability of the Q biosynthetic complex, is associated with a Coq6-containing complex. Additionally Q6 and late stage Q biosynthetic intermediates were also found to co-purify with the complex. A mitochondrial protein of unknown function, encoded by the YLR290C open reading frame, is also identified as a constituent of the complex and is shown to be required for efficient de novo Q biosynthesis. Finally, given its effect on Q synthesis and its association with the biosynthetic complex, we propose that the open reading frame YLR290C be designated COQ11.},
doi = {10.1074/jbc.m114.633131},
journal = {Journal of Biological Chemistry},
number = 12,
volume = 290,
place = {United States},
year = {Wed Jan 28 00:00:00 EST 2015},
month = {Wed Jan 28 00:00:00 EST 2015}
}

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