Enhanced triacylglycerol accumulation in vegetative tissues of plants

Xu, Changcheng; Fan, Jilian; Yan, Chengshi; Shanklin, John

Title: Enhanced triacylglycerol accumulation in vegetative tissues of plants

Patent · Tue Mar 14 00:00:00 EDT 2017

OSTI ID:1346973

Xu, Changcheng; Fan, Jilian; Yan, Chengshi; Shanklin, John

In the tgd1-1 mutant that displays substantially enhanced TAG synthesis and turnover, disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases FA turnover, leading to an increase in leaf TAG content up to 9% of dry weight and total leaf lipid by three-fold. The membrane lipid content and composition of tgd1-1 sdp1-4 and tgd1-1 pxa1-2 double mutants are altered and they are compromised in growth and development and fertility.

View Patent

Cite

Export

Save

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886; SC0012704

Assignee:: Brookhaven Science Associates, LLC

Patent Number(s):: 9,593,344

Application Number:: 14/790,139

OSTI ID:: 1346973

Resource Relation:: Patent File Date: 2015 Jul 02

Country of Publication:: United States

Language:: English

References (50)

Regulation of peroxisomal fatty acid transport in plants Baker, Alison; Slocombe, Stephen; Graham, Ian Alexander https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7626079 US Patent Document 7,626,079	patent	December 2009
Reduced RDM-1 gene expression in plants Eastmond, Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8093452 US Patent Document 8,093,452	patent	January 2012
The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis Kelly, A. A.; van Erp, H.; Quettier, A.-L. PLANT PHYSIOLOGY, Vol. 162, Issue 3, p. 1282-1289 https://doi.org/10.1104/pp.113.219840	journal	May 2013
Mutation of the TGD1 Chloroplast Envelope Protein Affects Phosphatidate Metabolism in Arabidopsis Xu, Changcheng; Fan, Jilian; Froehlich, John E. The Plant Cell, Vol. 17, Issue 11, p. 3094-3110 https://doi.org/10.1105/tpc.105.035592	journal	November 2005
The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets Adeyo, Oludotun; Horn, Patrick J.; Lee, SungKyung The Journal of Cell Biology, Vol. 192, Issue 6, p. 1043-1055 https://doi.org/10.1083/jcb.201010111	journal	March 2011
Overexpression of Diacylglycerol Acyltransferase-1 Reduces Phospholipid Synthesis, Proliferation, and Invasiveness in Simian Virus 40-transformed Human Lung Fibroblasts Bagnato, Carolina; Igal, R. Ariel Journal of Biological Chemistry, Vol. 278, Issue 52, p. 52203-52211 https://doi.org/10.1074/jbc.M305760200	journal	October 2003
Understanding in vivo carbon precursor supply for fatty acid synthesis in leaf tissue Bao, Xiaoming; Focke, Manfred; Pollard, Mike The Plant Journal, Vol. 22, Issue 1, p. 39-50 https://doi.org/10.1046/j.1365-313x.2000.00712.x	journal	April 2000
A Mutant of Arabidopsis Deficient in the Chloroplast 16:1/18:1 Desaturase Browse, J.; Kunst, L.; Anderson, S. Plant Physiology, Vol. 90, Issue 2, p. 522-529 https://doi.org/10.1104/pp.90.2.522	journal	June 1989
Phosphatidylcholine deficiency upregulates enzymes of triacylglycerol metabolism in CHO cells Caviglia, J. Matías; de Gómez Dumm, I. Nelva T.; Coleman, Rosalind A. Journal of Lipid Research, Vol. 45, Issue 8, p. 1500-1509 https://doi.org/10.1194/jlr.M400079-JLR200	journal	June 2004
Compartmentation of Triacylglycerol Accumulation in Plants Chapman, Kent D.; Ohlrogge, John B. Journal of Biological Chemistry, Vol. 287, Issue 4, p. 2288-2294 https://doi.org/10.1074/jbc.R111.290072	journal	November 2011
Phospholipid:diacylglycerol acyltransferase-mediated triacylglycerol biosynthesis is crucial for protection against fatty acid-induced cell death in growing tissues of Arabidopsis Fan, Jilian; Yan, Chengshi; Xu, Changcheng The Plant Journal, Vol. 76, Issue 6, p. 930-942 https://doi.org/10.1111/tpj.12343	journal	November 2013
Dual Role for Phospholipid:Diacylglycerol Acyltransferase: Enhancing Fatty Acid Synthesis and Diverting Fatty Acids from Membrane Lipids to Triacylglycerol in Arabidopsis Leaves Fan, J.; Yan, C.; Zhang, X. The Plant Cell, Vol. 25, Issue 9, p. 3506-3518 https://doi.org/10.1105/tpc.113.117358	journal	September 2013
A Block in Endoplasmic Reticulum-to-Golgi Trafficking Inhibits Phospholipid Synthesis and Induces Neutral Lipid Accumulation Gaspar, Maria L.; Jesch, Stephen A.; Viswanatha, Raghuvir Journal of Biological Chemistry, Vol. 283, Issue 37, p. 25735-25751 https://doi.org/10.1074/jbc.M802685200	journal	July 2008
At4g24160, a Soluble Acyl-Coenzyme A-Dependent Lysophosphatidic Acid Acyltransferase Ghosh, A. K.; Chauhan, N.; Rajakumari, S. Plant Physiology, Vol. 151, Issue 2, p. 869-881 https://doi.org/10.1104/pp.109.144261	journal	August 2009
Inactivation of the C. elegans lipin homolog leads to ER disorganization and to defects in the breakdown and reassembly of the nuclear envelope Golden, A.; Liu, J.; Cohen-Fix, O. Journal of Cell Science, Vol. 122, Issue 12, p. 1970-1978 https://doi.org/10.1242/jcs.044743	journal	June 2009
Seed Storage Oil Mobilization Graham, Ian A. Annual Review of Plant Biology, Vol. 59, Issue 1, p. 115-142 https://doi.org/10.1146/annurev.arplant.59.032607.092938	journal	June 2008
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation Hajdukiewicz, Peter; Svab, Zora; Maliga, Pal Plant Molecular Biology, Vol. 25, Issue 6, p. 989-994 https://doi.org/10.1007/BF00014672	journal	September 1994
DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis Hartel, H.; Dormann, P.; Benning, C. Proceedings of the National Academy of Sciences, Vol. 97, Issue 19, p. 10649-10654 https://doi.org/10.1073/pnas.180320497	journal	September 2000
Metabolism and Regulation of Glycerolipids in the Yeast Saccharomyces cerevisiae Henry, Susan A.; Kohlwein, Sepp D.; Carman, George M. Genetics, Vol. 190, Issue 2, p. 317-349 https://doi.org/10.1534/genetics.111.130286	journal	February 2012
Mechanisms of glycerophospholipid homeostasis in mammalian cells Hermansson, Martin; Hokynar, Kati; Somerharju, Pentti Progress in Lipid Research, Vol. 50, Issue 3, p. 240-257 https://doi.org/10.1016/j.plipres.2011.02.004	journal	July 2011
Lipid landscapes and pipelines in membrane homeostasis Holthuis, Joost C.M.; Menon, Anant K. Nature, Vol. 510, Issue 7503, p. 48-57 https://doi.org/10.1038/nature13474	journal	June 2014
Acylglycerol Recycling from Triacylglycerol to Phospholipid, Not Lipase Activity, Is Defective in Neutral Lipid Storage Disease Fibroblasts Igal, R. Ariel; Coleman, Rosalind A. Journal of Biological Chemistry, Vol. 271, Issue 28, p. 16644-16651 https://doi.org/10.1074/jbc.271.28.16644	journal	July 1996
Disruption of the Arabidopsis CGI-58 homologue produces Chanarin–Dorfman-like lipid droplet accumulation in plants James, Christopher N.; Horn, Patrick J.; Case, Charlene R. Proceedings of the National Academy of Sciences, Vol. 107, Issue 41, p. 17833-17838 https://doi.org/10.1073/pnas.0911359107	journal	September 2010
Seed Storage Oil Mobilization Is Important But Not Essential for Germination or Seedling Establishment in Arabidopsis Kelly, A. A.; Quettier, A.-L.; Shaw, E. PLANT PHYSIOLOGY, Vol. 157, Issue 2, p. 866-875 https://doi.org/10.1104/pp.111.181784	journal	October 2011
Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis Kobayashi, K.; Kondo, M.; Fukuda, H. Proceedings of the National Academy of Sciences, Vol. 104, Issue 43, p. 17216-17221 https://doi.org/10.1073/pnas.0704680104	journal	October 2007
Lipid Droplets and Peroxisomes: Key Players in Cellular Lipid Homeostasis or A Matter of Fat--Store 'em Up or Burn 'em Down Kohlwein, S. D.; Veenhuis, M.; van der Klei, I. J. Genetics, Vol. 193, Issue 1, p. 1-50 https://doi.org/10.1534/genetics.112.143362	journal	December 2012
Identification of a plastid acyl-acyl carrier protein synthetase in Arabidopsis and its role in the activation and elongation of exogenous fatty acids Koo, Abraham J. K.; Fulda, Martin; Browse, John The Plant Journal, Vol. 44, Issue 4, p. 620-632 https://doi.org/10.1111/j.1365-313X.2005.02553.x	journal	October 2005
Altered regulation of lipid biosynthesis in a mutant of Arabidopsis deficient in chloroplast glycerol-3-phosphate acyltransferase activity Kunst, L.; Browse, J.; Somerville, C. Proceedings of the National Academy of Sciences, Vol. 85, Issue 12, p. 4143-4147 https://doi.org/10.1073/pnas.85.12.4143	journal	June 1988
The ABC Transporter PXA1 and Peroxisomal -Oxidation Are Vital for Metabolism in Mature Leaves of Arabidopsis during Extended Darkness Kunz, H.-H.; Scharnewski, M.; Feussner, K. The Plant Cell Online, Vol. 21, Issue 9, p. 2733-2749 https://doi.org/10.1105/tpc.108.064857	journal	September 2009
Elimination of the CDP-ethanolamine Pathway Disrupts Hepatic Lipid Homeostasis Leonardi, Roberta; Frank, Matthew W.; Jackson, Pamela D. Journal of Biological Chemistry, Vol. 284, Issue 40, p. 27077-27089 https://doi.org/10.1074/jbc.M109.031336	journal	August 2009
S -Adenosyl-L-homocysteine Hydrolase, Key Enzyme of Methylation Metabolism, Regulates Phosphatidylcholine Synthesis and Triacylglycerol Homeostasis in Yeast : IMPLICATIONS FOR HOMOCYSTEINE AS A RISK FACTOR OF ATHEROSCLEROSIS Malanovic, Nermina; Streith, Ingo; Wolinski, Heimo Journal of Biological Chemistry, Vol. 283, Issue 35, p. 23989-23999 https://doi.org/10.1074/jbc.M800830200	journal	June 2008
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Murashige, Toshio; Skoog, Folke Physiologia Plantarum, Vol. 15, Issue 3, p. 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x	journal	July 1962
Arabidopsis lipins mediate eukaryotic pathway of lipid metabolism and cope critically with phosphate starvation Nakamura, Y.; Koizumi, R.; Shui, G. Proceedings of the National Academy of Sciences, Vol. 106, Issue 49, p. 20978-20983 https://doi.org/10.1073/pnas.0907173106	journal	November 2009
Coordination of Lipid Metabolism in Membrane Biogenesis Nohturfft, Axel; Zhang, Shao Chong Annual Review of Cell and Developmental Biology, Vol. 25, Issue 1, p. 539-566 https://doi.org/10.1146/annurev.cellbio.24.110707.175344	journal	November 2009
Lipid Biosynthesis Ohlrogge, J.; Browse, J. THE PLANT CELL ONLINE, Vol. 7, Issue 7, p. 957-970 https://doi.org/10.1105/tpc.7.7.957	journal	July 1995
The α/β Hydrolase CGI-58 and Peroxisomal Transport Protein PXA1 Coregulate Lipid Homeostasis and Signaling in Arabidopsis Park, Sunjung; Gidda, Satinder K.; James, Christopher N. The Plant Cell, Vol. 25, Issue 5, p. 1726-1739 https://doi.org/10.1105/tpc.113.111898	journal	May 2013
Good Fat, Essential Cellular Requirements for Triacylglycerol Synthesis to Maintain Membrane Homeostasis in Yeast Petschnigg, Julia; Wolinski, Heimo; Kolb, Dagmar Journal of Biological Chemistry, Vol. 284, Issue 45, p. 30981-30993 https://doi.org/10.1074/jbc.M109.024752	journal	July 2009
The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth Santos-Rosa, Helena; Leung, Joanne; Grimsey, Neil The EMBO Journal, Vol. 24, Issue 11, p. 1931-1941 https://doi.org/10.1038/sj.emboj.7600672	journal	May 2005
Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways Slocombe, Steve P.; Cornah, Jo; Pinfield-Wells, Helen Plant Biotechnology Journal, Vol. 7, Issue 7, p. 694-703 https://doi.org/10.1111/j.1467-7652.2009.00435.x	journal	September 2009
Seed storage oil catabolism: a story of give and take Theodoulou, Frederica L.; Eastmond, Peter J. Current Opinion in Plant Biology, Vol. 15, Issue 3, p. 322-328 https://doi.org/10.1016/j.pbi.2012.03.017	journal	June 2012
Lipid Trafficking between the Endoplasmic Reticulum and the Plastid in Arabidopsis Requires the Extraplastidic TGD4 Protein Xu, C.; Fan, J.; Cornish, A. J. The Plant Cell, Vol. 20, Issue 8, p. 2190-2204 https://doi.org/10.1105/tpc.108.061176	journal	August 2008
A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis Xu, C. The EMBO Journal, Vol. 22, Issue 10, p. 2370-2379 https://doi.org/10.1093/emboj/cdg234	journal	May 2003
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana Alonso, J. M.; Stepanova, Anna N.; Leisse, Thomas J. Science, Vol. 301, Issue 5633, p. 653-657 https://doi.org/10.1126/science.1086391	journal	August 2003
Metabolic Responses to the Reduction in Palmitate Caused by Disruption of the FATB Gene in Arabidopsis Bonaventure, Gustavo; Bao, Xiaoming; Ohlrogge, John Plant Physiology, Vol. 135, Issue 3, p. 1269-1279 https://doi.org/10.1104/pp.104.043372	journal	July 2004
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough, Steven J.; Bent, Andrew F. The Plant Journal, Vol. 16, Issue 6, p. 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x	journal	December 1998
Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids De Marcos Lousa, Carine; van Roermund, Carlo W. T.; Postis, Vincent L. G. Proceedings of the National Academy of Sciences, Vol. 110, Issue 4, p. 1279-1284 https://doi.org/10.1073/pnas.1218034110	journal	January 2013
Isolation and Characterization of an Arabidopsis Mutant Deficient in the Thylakoid Lipid Digalactosyl Diacylglycerol Dormann, P.; Hoffmann-Benning, S.; Balbo, I. The Plant Cell Online, Vol. 7, Issue 11, p. 1801-1810 https://doi.org/10.1105/tpc.7.11.1801	journal	November 1995
SUGAR-DEPENDENT1 Encodes a Patatin Domain Triacylglycerol Lipase That Initiates Storage Oil Breakdown in Germinating Arabidopsis Seeds Eastmond, P. J. The Plant Cell Online, Vol. 18, Issue 3, p. 665-675 https://doi.org/10.1105/tpc.105.040543	journal	March 2006
PHOSPHATIDIC ACID PHOSPHOHYDROLASE1 and 2 Regulate Phospholipid Synthesis at the Endoplasmic Reticulum in Arabidopsis Eastmond, P. J.; Quettier, A.-L.; Kroon, J. T. M. The Plant Cell, Vol. 22, Issue 8, p. 2796-2811 https://doi.org/10.1105/tpc.109.071423	journal	August 2010
A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii Fan, Jilian; Andre, Carl; Xu, Changcheng FEBS Letters, Vol. 585, Issue 12, p. 1985-1991 https://doi.org/10.1016/j.febslet.2011.05.018	journal	May 2011

Similar Records

Purple acid phosphatase2 stimulates a futile cycle of lipid synthesis and degradation, and mitigates the negative growth effects of triacylglycerol accumulation in vegetative tissues

Journal Article · Mon Jul 18 00:00:00 EDT 2022 · New Phytologist · OSTI ID:1346973

Cai, Yingqi; Zhai, Zhiyang; Blanford, Jantana; +5 more

Sugar Potentiation of Fatty Acid and Triacylglycerol Accumulation

Journal Article · Fri Aug 25 00:00:00 EDT 2017 · Plant Physiology (Bethesda) · OSTI ID:1346973

Zhai, Zhiyang; Liu, Hui; Xu, Changcheng; +1 more

Towards oilcane: Engineering hyperaccumulation of triacylglycerol into sugarcane stems

Journal Article · Sat May 23 00:00:00 EDT 2020 · Global Change Biology. Bioenergy · OSTI ID:1346973

Parajuli, Saroj; Kannan, Baskaran; Karan, Ratna; +9 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Enhanced triacylglycerol accumulation in vegetative tissues of plants

Citation Formats

References (50)

Similar Records

Related Subjects