Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy

Farahi, R. H.; Charrier, Anne M.; Tolbert, Allison K.; Lereu, Aude L.; Ragauskas, Arthur J.; Davison, Brian H.; Passian, Ali

doi:10.1038/s41598-017-00234-4

Title: Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy

Journal Article · Fri Mar 10 00:00:00 EST 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-00234-4· OSTI ID:1346628

Farahi, R. H. ^[1];

^[2]; Tolbert, Allison K. ^[3]; Lereu, Aude L. ^[4]; Ragauskas, Arthur J. ^[5]; Davison, Brian H. ^[5]; Passian, Ali ^[6]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Quantum Information Science, Computational Sciences and Engineering Division. BioEnergy Science Center (BESC). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Aix Marseille Univ., Marseille (France). Interdisciplinary Center of Nanoscience at Marseille (CINaM)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC). Biosciences Division; Georgia Inst. of Technology, Atlanta, GA (United States). School of Chemistry and Biochemistry
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Quantum Information Science, Computational Sciences and Engineering Division; Aix Marseille Univ., Marseille (France). Interdisciplinary Center of Nanoscience at Marseille (CINaM)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Quantum Information Science, Computational Sciences and Engineering Division. BioEnergy Science Center (BESC). Biosciences Division; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering. Dept. of Physics

The complex organic polymer, lignin, abundant in plants, prevents the efficient extraction of sugars from the cell walls that is required for large scale biofuel production. Because lignin removal is crucial in overcoming this challenge, the question of how the nanoscale properties of the plant cell ultrastructure correlate with delignification processes is important. Here, we report how distinct molecular domains can be identified and how physical quantities of adhesion energy, elasticity, and plasticity undergo changes, and whether such quantitative observations can be used to characterize delignification. By chemically processing biomass, and employing nanometrology, the various stages of lignin removal are shown to be distinguished through the observed morphochemical and nanomechanical variations. Such spatially resolved correlations between chemistry and nanomechanics during deconstruction not only provide a better understanding of the cell wall architecture but also is vital for devising optimum chemical treatments.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Univ. of Tennessee, Knoxville, TN (United States); Aix Marseille Univ., Marseille (France); Georgia Inst. of Technology, Atlanta, GA (United States)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1346628

Journal Information:: Scientific Reports, Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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