Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

Frontalini, Fabrizio; Curzi, Davide; Cesarini, Erica; Canonico, Barbara; Giordano, Francesco M.; De Matteis, Rita; Bernhard, Joan M.; Pieretti, Nadia; Gu, Baohua; Eskelsen, Jeremy R.; Jubb, Aaron M.; Zhao, Linduo; Pierce, Eric M.; Gobbi, Pietro; Papa, Stefano; Coccioni, Rodolfo; Hu, ed., Yi

doi:10.1371/journal.pone.0162401

Title: Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

Journal Article · Wed Sep 07 00:00:00 EDT 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0162401· OSTI ID:1346610

; Curzi, Davide; Cesarini, Erica; Canonico, Barbara; Giordano, Francesco M.; De Matteis, Rita; Bernhard, Joan M.; Pieretti, Nadia; Gu, Baohua; Eskelsen, Jeremy R.; Jubb, Aaron M.; Zhao, Linduo; Pierce, Eric M.; Gobbi, Pietro; Papa, Stefano; Coccioni, Rodolfo; Hu, ed., Yi

In this study, heavy metals such as mercury (Hg) pose a significant health hazard through bioaccumulation and biomagnification. By penetrating cell membranes, heavy metal ions may lead to pathological conditions. Here we examined the responses of Ammonia parkinsoniana, a benthic foraminiferan, to different concentrations of Hg in the artificial sea water. Confocal images of untreated and treated specimens using fluorescent probes (Nile Red and Acridine Orange) provided an opportunity for visualizing the intracellular lipid accumulation and acidic compartment regulation. With increased Hg over time, we observed an increased number of lipid droplets, which may have acted as a detoxifying organelle where Hg is sequestered and biologically inactivated. Further, Hg seems to promote the proliferation of lysosomes both in terms of number and dimension that, at the highest level of Hg, resulted in cell death. We report, for the first time, the presence of Hg within the foraminiferal cell: at the basal part of pores, in the organic linings of the foramen/septa, and as cytoplasmic accumulations.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1346610

Alternate ID(s):: OSTI ID: 1324211

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Vol. 11 Journal Issue: 9; ISSN 1932-6203

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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59 BASIC BIOLOGICAL SCIENCES
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Title: Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

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