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Title: Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.

Inventors:
; ;
Publication Date:
Research Org.:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1346066
Patent Number(s):
9,587,252
Application Number:
14/674,895
Assignee:
President and Fellows of Harvard College CHO
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Mar 31
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M., Esvelt, Kevin, and Mali, Prashant. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. United States: N. p., 2017. Web.
Church, George M., Esvelt, Kevin, & Mali, Prashant. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. United States.
Church, George M., Esvelt, Kevin, and Mali, Prashant. Tue . "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing". United States. doi:. https://www.osti.gov/servlets/purl/1346066.
@article{osti_1346066,
title = {Orthogonal Cas9 proteins for RNA-guided gene regulation and editing},
author = {Church, George M. and Esvelt, Kevin and Mali, Prashant},
abstractNote = {Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 07 00:00:00 EST 2017},
month = {Tue Mar 07 00:00:00 EST 2017}
}

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Works referenced in this record:

Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism in prokaryotes
journal, July 2011

  • Al-Attar, Sinan; Westra, Edze R.; van der Oost, John
  • Biological Chemistry, Vol. 392, Issue 4, p. 277-289
  • DOI: 10.1515/bc.2011.042

A CRISPR Approach to Gene Targeting
journal, September 2012


Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013


Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
journal, September 2012

  • Gasiunas, G.; Barrangou, R.; Horvath, P.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 39, p. E2579-E2586
  • DOI: 10.1073/pnas.1208507109

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
journal, July 2013


Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs
journal, February 2012


Mature clustered, regularly interspaced, short palindromic repeats RNA (crRNA) length is measured by a ruler mechanism anchored at the precursor processing site
journal, December 2011

  • Hatoum-Aslan, A.; Maniv, I.; Marraffini, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 52, p. 21218-21222
  • DOI: 10.1073/pnas.1112832108

RNA-programmed genome editing in human cells
journal, January 2013

  • Jinek, Martin; East, Alexandra; Cheng, Aaron
  • eLife, Vol. 2, Article No. e00471
  • DOI: 10.7554/eLife.00471

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
journal, June 2012


Evolution and classification of the CRISPR–Cas systems
journal, May 2011

  • Makarova, Kira S.; Haft, Daniel H.; Barrangou, Rodolphe
  • Nature Reviews Microbiology, Vol. 9, Issue 6, p. 467-477
  • DOI: 10.1038/nrmicro2577

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
journal, February 2013


Diverse CRISPRs Evolving in Human Microbiomes
journal, June 2012


RNA-guided genetic silencing systems in bacteria and archaea
journal, February 2012

  • Wiedenheft, Blake; Sternberg, Samuel H.; Doudna, Jennifer A.
  • Nature, Vol. 482, Issue 7385, p. 331-338
  • DOI: 10.1038/nature10886