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Title: Compositions for saccharification of cellulosic material

Abstract

The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.

Inventors:
; ;
Publication Date:
Research Org.:
Novozymes, Inc. Davis, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1346062
Patent Number(s):
9,587,262
Application Number:
14/885,555
Assignee:
Novozymes, Inc. GFO
DOE Contract Number:
FC36-08GO18080
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Oct 16
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

McBrayer, Brett, Shaghasi, Tarana, and Vlasenko, Elena. Compositions for saccharification of cellulosic material. United States: N. p., 2017. Web.
McBrayer, Brett, Shaghasi, Tarana, & Vlasenko, Elena. Compositions for saccharification of cellulosic material. United States.
McBrayer, Brett, Shaghasi, Tarana, and Vlasenko, Elena. Tue . "Compositions for saccharification of cellulosic material". United States. doi:. https://www.osti.gov/servlets/purl/1346062.
@article{osti_1346062,
title = {Compositions for saccharification of cellulosic material},
author = {McBrayer, Brett and Shaghasi, Tarana and Vlasenko, Elena},
abstractNote = {The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 07 00:00:00 EST 2017},
month = {Tue Mar 07 00:00:00 EST 2017}
}

Patent:

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  • The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
  • The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
  • The present invention relates to compositions comprising a GH61 polypeptide having cellulolytic enhancing activity and an organic compound comprising a carboxylic acid moiety, a lactone moiety, a phenolic moiety, a flavonoid moiety, or a combination thereof, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the organic compound enhances hydrolysis of a cellulosic material by a cellulolytic enzyme compared to the GH61 polypeptide alone or the organic compound alone. The present invention also relates to methods of using the compositions.
  • The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.
  • Transgenic lignocellulosic plants are provided according to embodiments of the present invention, the transgenic plants transformed with an expression cassette encoding a protein operably linked to a signal peptide which targets the protein to a cell wall of the transgenic plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine. Methods of increasing lignin-protein bonds in a lignocellulosic plant are provided according to embodiments of the present invention which include expressing a recombinant nucleic acid in a lignocellulosic plant, the recombinant nucleic acid encoding a protein operably linked tomore » a signal peptide which targets the protein to the cell wall of a plant, where at least 5% of the total amino acid residues of the protein are tyrosine, lysine, serine, threonine or cysteine.« less