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Title: Expanding the eukaryotic genetic code

Patent ·
OSTI ID:1345382
; ; ;

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

Research Organization:
The Scripps Reserach Institute, La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Reserach Institute
Patent Number(s):
9,580,721
Application Number:
14/574,257
OSTI ID:
1345382
Resource Relation:
Patent File Date: 2014 Dec 17
Country of Publication:
United States
Language:
English

References (144)

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Expanding the eukaryotic genetic code patent October 2009
Expanding the eukaryotic genetic code patent November 2009
Expanding the eukaryotic genetic code patent December 2009
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent December 2009
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Expanding the eukaryotic genetic code patent September 2010
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Expanding the eukaryotic genetic code patent-application January 2005
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application September 2005
Site-specific incorporation of redox active amino acids into proteins patent-application October 2005
In vivo incorporation of unnatural amino acids patent-application November 2005
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination patent-application December 2005
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In vivo incorporation of unnatural amino acids patent-application July 2008
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Expanding the eukaryotic genetic code patent-application July 2008
Glycoprotein synthesis patent-application September 2008
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Site specific incorporation of keto amino acids into proteins patent-application September 2008
Expanding the eukaryotic genetic code patent-application September 2008
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In vivo site-sepecific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent-application October 2008
In vivo incorporation of unnatural amino acids patent-application October 2008
Methods and composition for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent-application February 2009
In vivo incorporation of unnatural amino acids patent-application March 2009
Site-specific labeling of proteins for NMR studies patent-application March 2009
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