skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis

Abstract

Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologically important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.

Authors:
 [1];  [2];  [2];  [2];  [2];  [2];  [2];  [1];  [1];  [2];  [3];  [4]
  1. Northwestern Univ., Evanston, IL (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
  3. Northwestern Univ., Evanston, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States)
  4. Irvine (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH) - National Institute of General Medical Sciences; National Institutes of Health (NIH)
OSTI Identifier:
1344551
Alternate Identifier(s):
OSTI ID: 1194125; OSTI ID: 1396030
Grant/Contract Number:
AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 10; Journal Issue: 2; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; liquid nitrogen; fluorescence microscopy; fluorescence imaging; ultraviolet radiation; X-ray radiography; X-ray microscopy; dehydration (medicine); fibroblasts

Citation Formats

Jin, Qiaoling, Vogt, Stefan, Lai, Barry, Chen, Si, Finney, Lydia, Gleber, Sophie-Charlotte, Ward, Jesse, Deng, Junjing, Mak, Rachel, Moonier, Nena, Jacobsen, Chris, and Brody, James P. Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. United States: N. p., 2015. Web. doi:10.1371/journal.pone.0117437.
Jin, Qiaoling, Vogt, Stefan, Lai, Barry, Chen, Si, Finney, Lydia, Gleber, Sophie-Charlotte, Ward, Jesse, Deng, Junjing, Mak, Rachel, Moonier, Nena, Jacobsen, Chris, & Brody, James P. Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis. United States. doi:10.1371/journal.pone.0117437.
Jin, Qiaoling, Vogt, Stefan, Lai, Barry, Chen, Si, Finney, Lydia, Gleber, Sophie-Charlotte, Ward, Jesse, Deng, Junjing, Mak, Rachel, Moonier, Nena, Jacobsen, Chris, and Brody, James P. Mon . "Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis". United States. doi:10.1371/journal.pone.0117437. https://www.osti.gov/servlets/purl/1344551.
@article{osti_1344551,
title = {Ultraviolet germicidal irradiation and its effects on elemental distributions in mouse embryonic fibroblast cells in x-ray fluorescence microanalysis},
author = {Jin, Qiaoling and Vogt, Stefan and Lai, Barry and Chen, Si and Finney, Lydia and Gleber, Sophie-Charlotte and Ward, Jesse and Deng, Junjing and Mak, Rachel and Moonier, Nena and Jacobsen, Chris and Brody, James P.},
abstractNote = {Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated with Risk Group 2 biological materials, one must be concerned with contamination of expensive and complicated cryogenic x-ray microscopes when working with such materials. We employed ultraviolet germicidal irradiation to decontaminate previously cryopreserved cells under liquid nitrogen, and then investigated its effects on elemental distributions under both frozen hydrated and freeze dried states with x-ray fluorescence microscopy. We show that the contents and distributions of most biologically important elements remain nearly unchanged when compared with non-ultraviolet-irradiated counterparts, even after multiple cycles of ultraviolet germicidal irradiation and cryogenic x-ray imaging. This provides a potential pathway for rendering Risk Group 2 biological materials safe for handling in multiuser cryogenic x-ray microscopes without affecting the fidelity of the results.},
doi = {10.1371/journal.pone.0117437},
journal = {PLoS ONE},
number = 2,
volume = 10,
place = {United States},
year = {Mon Feb 23 00:00:00 EST 2015},
month = {Mon Feb 23 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 7 works
Citation information provided by
Web of Science

Save / Share: