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Title: Systems and methods for the secretion of recombinant proteins in gram negative bacteria

Abstract

Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.

Inventors:
; ; ;
Publication Date:
Research Org.:
WISCONSIN ALUMNI RESEARCH FOUNDATION, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1344502
Patent Number(s):
9,574,197
Application Number:
15/211,632
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION CHO
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 Jul 15
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Withers, III, Sydnor T., Dominguez, Miguel A., DeLisa, Matthew P., and Haitjema, Charles H. Systems and methods for the secretion of recombinant proteins in gram negative bacteria. United States: N. p., 2017. Web.
Withers, III, Sydnor T., Dominguez, Miguel A., DeLisa, Matthew P., & Haitjema, Charles H. Systems and methods for the secretion of recombinant proteins in gram negative bacteria. United States.
Withers, III, Sydnor T., Dominguez, Miguel A., DeLisa, Matthew P., and Haitjema, Charles H. Tue . "Systems and methods for the secretion of recombinant proteins in gram negative bacteria". United States. doi:. https://www.osti.gov/servlets/purl/1344502.
@article{osti_1344502,
title = {Systems and methods for the secretion of recombinant proteins in gram negative bacteria},
author = {Withers, III, Sydnor T. and Dominguez, Miguel A. and DeLisa, Matthew P. and Haitjema, Charles H.},
abstractNote = {Disclosed herein are systems and methods for producing recombinant proteins utilizing mutant E. coli strains containing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the culture medium. Host cells transformed with the expression vectors are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 21 00:00:00 EST 2017},
month = {Tue Feb 21 00:00:00 EST 2017}
}

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Works referenced in this record:

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