skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Abstract

In this paper, recombinant spider silks produced in transgenic goat milk were studied as cell culture matrices for neuronal growth. Major ampullate spidroin 1 (MaSp1) supported neuronal growth, axon extension and network connectivity, with cell morphology comparable to the gold standard poly-lysine. In addition, neurons growing on MaSp1 films had increased neural cell adhesion molecule (NCAM) expression at both mRNA and protein levels. The results indicate that MaSp1 films present useful surface charge and substrate stiffness to support the growth of primary rat cortical neurons. Moreover, a putative neuron-specific surface binding sequence GRGGL within MaSp1 may contribute to the biological regulation of neuron growth. These findings indicate that MaSp1 could regulate neuron growth through its physical and biological features. Finally, this dual regulation mode of MaSp1 could provide an alternative strategy for generating functional silk materials for neural tissue engineering.

Authors:
 [1];  [1];  [1];  [1];  [2];  [2];  [1]
  1. Tufts Univ., Medford, MA (United States). Dept. of Biomedical Engineering
  2. Utah State Univ., Logan, UT (United States). Dept. of Biology
Publication Date:
Research Org.:
Utah State Univ., Logan, UT (United States); Tufts Univ., Medford, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC): National Institutes of Health (NIH); USDOE
OSTI Identifier:
1344119
Alternate Identifier(s):
OSTI ID: 1365582
Grant/Contract Number:
SC0004791; IIP-1318194; R01 EB014283; P41 EB002520; R01 EY020856
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biomaterials
Additional Journal Information:
Journal Volume: 48; Journal ID: ISSN 0142-9612
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Biomaterial; Neural cell; Silk; Recombinant protein; Tissue engineering

Citation Formats

An, Bo, Tang-Schomer, Min D., Huang, Wenwen, He, Jiuyang, Jones, Justin A., Lewis, Randolph V., and Kaplan, David L. Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks. United States: N. p., 2015. Web. doi:10.1016/j.biomaterials.2015.01.044.
An, Bo, Tang-Schomer, Min D., Huang, Wenwen, He, Jiuyang, Jones, Justin A., Lewis, Randolph V., & Kaplan, David L. Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks. United States. doi:10.1016/j.biomaterials.2015.01.044.
An, Bo, Tang-Schomer, Min D., Huang, Wenwen, He, Jiuyang, Jones, Justin A., Lewis, Randolph V., and Kaplan, David L. Wed . "Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks". United States. doi:10.1016/j.biomaterials.2015.01.044. https://www.osti.gov/servlets/purl/1344119.
@article{osti_1344119,
title = {Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks},
author = {An, Bo and Tang-Schomer, Min D. and Huang, Wenwen and He, Jiuyang and Jones, Justin A. and Lewis, Randolph V. and Kaplan, David L.},
abstractNote = {In this paper, recombinant spider silks produced in transgenic goat milk were studied as cell culture matrices for neuronal growth. Major ampullate spidroin 1 (MaSp1) supported neuronal growth, axon extension and network connectivity, with cell morphology comparable to the gold standard poly-lysine. In addition, neurons growing on MaSp1 films had increased neural cell adhesion molecule (NCAM) expression at both mRNA and protein levels. The results indicate that MaSp1 films present useful surface charge and substrate stiffness to support the growth of primary rat cortical neurons. Moreover, a putative neuron-specific surface binding sequence GRGGL within MaSp1 may contribute to the biological regulation of neuron growth. These findings indicate that MaSp1 could regulate neuron growth through its physical and biological features. Finally, this dual regulation mode of MaSp1 could provide an alternative strategy for generating functional silk materials for neural tissue engineering.},
doi = {10.1016/j.biomaterials.2015.01.044},
journal = {Biomaterials},
number = ,
volume = 48,
place = {United States},
year = {Wed Feb 11 00:00:00 EST 2015},
month = {Wed Feb 11 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 8 works
Citation information provided by
Web of Science

Save / Share: