Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

An, Bo; Tang-Schomer, Min D.; Huang, Wenwen; He, Jiuyang; Jones, Justin A.; Lewis, Randolph V.; Kaplan, David L.

doi:10.1016/j.biomaterials.2015.01.044

Title: Physical and biological regulation of neuron regenerative growth and network formation on recombinant dragline silks

Journal Article · Wed Feb 11 00:00:00 EST 2015 · Biomaterials

DOI:https://doi.org/10.1016/j.biomaterials.2015.01.044· OSTI ID:1344119

An, Bo ^[1]; Tang-Schomer, Min D. ^[1]; Huang, Wenwen ^[1]; He, Jiuyang ^[1]; Jones, Justin A. ^[2]; Lewis, Randolph V. ^[2]; Kaplan, David L. ^[1]

Tufts Univ., Medford, MA (United States). Dept. of Biomedical Engineering
Utah State Univ., Logan, UT (United States). Dept. of Biology

In this paper, recombinant spider silks produced in transgenic goat milk were studied as cell culture matrices for neuronal growth. Major ampullate spidroin 1 (MaSp1) supported neuronal growth, axon extension and network connectivity, with cell morphology comparable to the gold standard poly-lysine. In addition, neurons growing on MaSp1 films had increased neural cell adhesion molecule (NCAM) expression at both mRNA and protein levels. The results indicate that MaSp1 films present useful surface charge and substrate stiffness to support the growth of primary rat cortical neurons. Moreover, a putative neuron-specific surface binding sequence GRGGL within MaSp1 may contribute to the biological regulation of neuron growth. These findings indicate that MaSp1 could regulate neuron growth through its physical and biological features. Finally, this dual regulation mode of MaSp1 could provide an alternative strategy for generating functional silk materials for neural tissue engineering.

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Research Organization:: Utah State Univ., Logan, UT (United States); Tufts Univ., Medford, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC): National Institutes of Health (NIH); USDOE

Grant/Contract Number:: SC0004791; IIP-1318194; R01 EB014283; P41 EB002520; R01 EY020856

OSTI ID:: 1344119

Alternate ID(s):: OSTI ID: 1365582

Journal Information:: Biomaterials, Vol. 48; ISSN 0142-9612

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 33 works

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