The PufX quinone channel enables the light‐harvesting 1 antenna to bind more carotenoids for light collection and photoprotection

Olsen, John D.; Martin, Elizabeth C.; Hunter, C. Neil

doi:10.1002/1873-3468.12575

Title: The PufX quinone channel enables the light‐harvesting 1 antenna to bind more carotenoids for light collection and photoprotection

Journal Article · Fri Feb 10 00:00:00 EST 2017 · FEBS Letters

DOI:https://doi.org/10.1002/1873-3468.12575· OSTI ID:1343454

Olsen, John D. ^[1]; Martin, Elizabeth C. ^[1]; Hunter, C. Neil ^[1]

Department of Molecular Biology and Biotechnology University of Sheffield UK

Photosynthesis in some phototrophic bacteria requires the PufX component of the reaction centre–light‐harvesting 1–PufX ( RC ‐ LH 1‐PufX) complex, which creates a pore for quinone/quinol (Q/ QH ₂ ) exchange across the LH 1 barrier surrounding the RC . However, photosynthetic bacteria such as Thermochromatium (T.) tepidum do not require PufX because there are fewer carotenoid binding sites, which creates multiple pores in the LH 1 ring for Q/ QH ₂ exchange. We show that an αTrp _‐24 →Phe alteration of the Rhodobacter (Rba.) sphaeroides LH 1 antenna impairs carotenoid binding and allows photosynthetic growth in the absence of PufX. We propose that acquisition of PufX and confining Q/ QH ₂ traffic to a pore adjacent to the RC Q _B site is an evolutionary upgrade that allows increased LH 1 carotenoid content for enhanced light absorption and photoprotection.

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Sponsoring Organization:: USDOE

OSTI ID:: 1343454

Alternate ID(s):: OSTI ID: 1343455

Journal Information:: FEBS Letters, Journal Name: FEBS Letters Vol. 591 Journal Issue: 4; ISSN 0014-5793

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 16 works

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