Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds

Kim, Hae Jin; Silva, Jillian E.; Vu, Hieu Sy; Mockaitis, Keithanne; Nam, Jeong -Won; Cahoon, Edgar B.

doi:10.1093/jxb/erv225

Title: Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds

Journal Article · Mon May 11 00:00:00 EDT 2015 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/erv225· OSTI ID:1343284

Kim, Hae Jin ^[1]; Silva, Jillian E. ^[1]; Vu, Hieu Sy ^[1]; Mockaitis, Keithanne ^[2]; Nam, Jeong -Won ^[3]; Cahoon, Edgar B. ^[1]

Univ. of Nebraska-Lincoln, Lincoln, NE (United States)
Indiana Univ., Bloomington, IN (United States)
Donald Danforth Plant Science Center, Saint Louis, MO (United States)

Seeds of members of the genus Cuphea accumulate medium-chain fatty acids (MCFAs; 8:0–14:0). MCFA- and palmitic acid- (16:0) rich vegetable oils have received attention for jet fuel production, given their similarity in chain length to Jet A fuel hydrocarbons. Studies were conducted to test genes, including those from Cuphea, for their ability to confer jet fuel-type fatty acid accumulation in seed oil of the emerging biofuel crop Camelina sativa. Transcriptomes from Cuphea viscosissima and Cuphea pulcherrima developing seeds that accumulate >90% of C8 and C10 fatty acids revealed three FatB cDNAs (CpuFatB3, CvFatB1, and CpuFatB4) expressed predominantly in seeds and structurally divergent from typical FatB thioesterases that release 16:0 from acyl carrier protein (ACP). Expression of CpuFatB3 and CvFatB1 resulted in Camelina oil with capric acid (10:0), and CpuFatB4 expression conferred myristic acid (14:0) production and increased 16:0. Co-expression of combinations of previously characterized Cuphea and California bay FatBs produced Camelina oils with mixtures of C8–C16 fatty acids, but amounts of each fatty acid were less than obtained by expression of individual FatB cDNAs. Increases in lauric acid (12:0) and 14:0, but not 10:0, in Camelina oil and at the sn-2 position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs. RNA interference (RNAi) suppression of Camelina β-ketoacyl-ACP synthase II, however, reduced 12:0 in seeds expressing a 12:0-ACP-specific FatB. Here, Camelina lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.

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Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Advanced Biofuel Systems (CABS); Univ. of of Nebraska-Lincoln, Lincoln, NE (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001295

OSTI ID:: 1343284

Journal Information:: Journal of Experimental Botany, Vol. 66, Issue 14; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 54 works

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60 APPLIED LIFE SCIENCES
Camelina
Cuphea
FatB acyl-ACP thioesterase
jet fuel oilseed
medium-chain fatty acid

Title: Toward production of jet fuel functionality in oilseeds: Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in Camelina seeds

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Cited By (16)

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