Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

Feenstra, Adam D.; Dueñas, Maria Emilia; Lee, Young Jin

doi:10.1007/s13361-016-1577-8

Title: Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

Journal Article · Tue Jan 03 00:00:00 EST 2017 · Journal of the American Society for Mass Spectrometry

DOI:https://doi.org/10.1007/s13361-016-1577-8· OSTI ID:1342921

Feenstra, Adam D. ^[1]; Dueñas, Maria Emilia ^[1];

^[1]

Ames Lab. and Iowa State Univ., Ames, IA (United States). Dept. of Chemistry

High-spatial resolution mass spectrometry imaging (MSI) is crucial for the mapping of chemical distributions at the cellular and subcellular level. Here in this work, we improved our previous laser optical system for matrix-assisted laser desorption ionization (MALDI)-MSI, from ~9 μm practical laser spot size to a practical laser spot size of ~4 μm, thereby allowing for 5 μm resolution imaging without oversampling. This is accomplished through a combination of spatial filtering, beam expansion, and reduction of the final focal length. Most importantly, the new laser optics system allows for simple modification of the spot size solely through the interchanging of the beam expander component. Using 10×, 5×, and no beam expander, we could routinely change between ~4, ~7, and ~45 μm laser spot size, in less than 5 min. We applied this multi-resolution MALDI-MSI system to a single maize root tissue section with three different spatial resolutions of 5, 10, and 50 μm and compared the differences in imaging quality and signal sensitivity. Lastly, we also demonstrated the difference in depth of focus between the optical systems with 10× and 5× beam expanders.

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Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1342921

Report Number(s):: IS-J-9151; PII: 1577

Journal Information:: Journal of the American Society for Mass Spectrometry, Vol. 28, Issue 3; ISSN 1044-0305

Publisher:: American Society for Mass SpectrometryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 63 works

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Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation Bowman, Andrew P.; Bogie, Jeroen F. J.; Hendriks, Jerome J. A. Analytical and Bioanalytical Chemistry, Vol. 412, Issue 10 https://doi.org/10.1007/s00216-019-02290-3	journal	December 2019
Specific Lipid and Metabolic Profiles of R-CHOP-Resistant Diffuse Large B-Cell Lymphoma Elucidated by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging and in Vivo Imaging Barré, Florian P. Y.; Claes, Britt S. R.; Dewez, Frédéric Analytical Chemistry, Vol. 90, Issue 24 https://doi.org/10.1021/acs.analchem.8b02910	journal	November 2018

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Title: Five Micron High Resolution MALDI Mass Spectrometry Imaging with Simple, Interchangeable, Multi-Resolution Optical System

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