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Title: Reviving Antibiotics: Efflux Pump Inhibitors That Interact with AcrA, a Membrane Fusion Protein of the AcrAB-TolC Multidrug Efflux Pump

Abstract

Antibiotic resistance is a major threat to human welfare. Inhibitors of multidrug efflux pumps (EPIs) are promising alternative therapeutics that could revive activities of antibiotics and reduce bacterial virulence. Identification of new druggable sites for inhibition is critical for developing effective EPIs, especially in light of constantly emerging resistance. We describe new EPIs that interact with and possibly inhibit the function of periplasmic membrane fusion proteins, critical components of efflux pumps that are responsible for the activation of the transporter and the recruitment of the outer-membrane channel. The discovered EPIs bind to AcrA, a component of the prototypical AcrAB-TolC pump, change its structure in vivo, inhibit efflux of fluorescent probes and potentiate the activities of antibiotics in Escherichia coli cells. These findings expand the chemical and mechanistic diversity of EPIs, suggest the mechanism for regulation of the efflux pump assembly and activity, and provide a promising path for reviving the activities of antibiotics in resistant bacteria.

Authors:
 [1];  [2];  [3];  [1];  [4];  [1];  [3];  [1];  [2];  [2];  [1]
  1. Univ. of Oklahoma, Norman, OK (United States). Dept. of Chemistry and Biochemistry
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biochemistry and Cellular and Molecular Biology
  3. Saint Louis Univ. School of Medicine, MS (United States). Dept. of Pharmacological and Physiological Science
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Molecular Biophysics
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1341559
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
ACS Infectious Diseases
Additional Journal Information:
Journal Volume: 3; Journal Issue: 1; Journal ID: ISSN 2373-8227
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; Titan

Citation Formats

Abdali, Narges, Parks, Jerry M., Haynes, Keith M., Chaney, Julie L., Green, Adam T., Wolloscheck, David, Walker, John K., Rybenkov, Valentin V., Baudry, Jerome, Smith, Jeremy C., and Zgurskaya, Helen I. Reviving Antibiotics: Efflux Pump Inhibitors That Interact with AcrA, a Membrane Fusion Protein of the AcrAB-TolC Multidrug Efflux Pump. United States: N. p., 2016. Web. doi:10.1021/acsinfecdis.6b00167.
Abdali, Narges, Parks, Jerry M., Haynes, Keith M., Chaney, Julie L., Green, Adam T., Wolloscheck, David, Walker, John K., Rybenkov, Valentin V., Baudry, Jerome, Smith, Jeremy C., & Zgurskaya, Helen I. Reviving Antibiotics: Efflux Pump Inhibitors That Interact with AcrA, a Membrane Fusion Protein of the AcrAB-TolC Multidrug Efflux Pump. United States. https://doi.org/10.1021/acsinfecdis.6b00167
Abdali, Narges, Parks, Jerry M., Haynes, Keith M., Chaney, Julie L., Green, Adam T., Wolloscheck, David, Walker, John K., Rybenkov, Valentin V., Baudry, Jerome, Smith, Jeremy C., and Zgurskaya, Helen I. 2016. "Reviving Antibiotics: Efflux Pump Inhibitors That Interact with AcrA, a Membrane Fusion Protein of the AcrAB-TolC Multidrug Efflux Pump". United States. https://doi.org/10.1021/acsinfecdis.6b00167. https://www.osti.gov/servlets/purl/1341559.
@article{osti_1341559,
title = {Reviving Antibiotics: Efflux Pump Inhibitors That Interact with AcrA, a Membrane Fusion Protein of the AcrAB-TolC Multidrug Efflux Pump},
author = {Abdali, Narges and Parks, Jerry M. and Haynes, Keith M. and Chaney, Julie L. and Green, Adam T. and Wolloscheck, David and Walker, John K. and Rybenkov, Valentin V. and Baudry, Jerome and Smith, Jeremy C. and Zgurskaya, Helen I.},
abstractNote = {Antibiotic resistance is a major threat to human welfare. Inhibitors of multidrug efflux pumps (EPIs) are promising alternative therapeutics that could revive activities of antibiotics and reduce bacterial virulence. Identification of new druggable sites for inhibition is critical for developing effective EPIs, especially in light of constantly emerging resistance. We describe new EPIs that interact with and possibly inhibit the function of periplasmic membrane fusion proteins, critical components of efflux pumps that are responsible for the activation of the transporter and the recruitment of the outer-membrane channel. The discovered EPIs bind to AcrA, a component of the prototypical AcrAB-TolC pump, change its structure in vivo, inhibit efflux of fluorescent probes and potentiate the activities of antibiotics in Escherichia coli cells. These findings expand the chemical and mechanistic diversity of EPIs, suggest the mechanism for regulation of the efflux pump assembly and activity, and provide a promising path for reviving the activities of antibiotics in resistant bacteria.},
doi = {10.1021/acsinfecdis.6b00167},
url = {https://www.osti.gov/biblio/1341559}, journal = {ACS Infectious Diseases},
issn = {2373-8227},
number = 1,
volume = 3,
place = {United States},
year = {Fri Oct 21 00:00:00 EDT 2016},
month = {Fri Oct 21 00:00:00 EDT 2016}
}

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Inhibiting Bacterial Drug Efflux Pumps via Phyto-Therapeutics to Combat Threatening Antimicrobial Resistance
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Drug combinations: a strategy to extend the life of antibiotics in the 21st century
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Permeability barriers of Gram‐negative pathogens
journal, June 2019


2038 – When microbes rule the Earth
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Inhibiting Bacterial Drug Efflux Pumps via Phyto-Therapeutics to Combat Threatening Antimicrobial Resistance
journal, December 2018


Molecules that Inhibit Bacterial Resistance Enzymes
journal, December 2018