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Title: A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks

Abstract

A new co-cultivation technology is presented that converts greenhouse gasses, CH 4 and CO 2, into microbial biomass. The methanotrophic bacterium, Methylomicrobium alcaliphilum 20z, was coupled to a cyanobacterium, Synechococcus PCC 7002 via oxygenic photosynthesis. The system exhibited robust growth on diverse gas mixtures ranging from biogas to those representative of a natural gas feedstock. A continuous processes was developed on a synthetic natural gas feed that achieved steady-state by imposing coupled light and O 2 limitations on the cyanobacterium and methanotroph, respectively. Continuous co-cultivation resulted in an O 2 depleted reactor and does not require CH 4/O 2 mixtures to be fed into the system, thereby enhancing process safety considerations over traditional methanotroph mono-culture platforms. This co-culture technology is scalable with respect to its ability to utilize different gas streams and its biological components constructed from model bacteria that can be metabolically customized to produce a range of biofuels and bioproducts.

Authors:
 [1];  [1];  [1];  [2]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division; Washington State Univ., Pullman, WA (United States). The Gene and Linda Voiland School of Chemical Engineering and Bioengineering
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1341100
Alternate Identifier(s):
OSTI ID: 1341742; OSTI ID: 1347845
Report Number(s):
PNNL-SA-122746
Journal ID: ISSN 0960-8524; PII: S0960852417300056
Grant/Contract Number:
AC05-76RL01830
Resource Type:
Journal Article: Published Article
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 228; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; methane; carbon dioxide; co-culture; biogas; natural gas; methanotroph; cyanobacteria; Bacteria; Environmental Molecular Sciences Laboratory

Citation Formats

Hill, Eric A., Chrisler, William B., Beliaev, Alex S., and Bernstein, Hans C. A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks. United States: N. p., 2017. Web. doi:10.1016/j.biortech.2016.12.111.
Hill, Eric A., Chrisler, William B., Beliaev, Alex S., & Bernstein, Hans C. A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks. United States. doi:10.1016/j.biortech.2016.12.111.
Hill, Eric A., Chrisler, William B., Beliaev, Alex S., and Bernstein, Hans C. Tue . "A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks". United States. doi:10.1016/j.biortech.2016.12.111.
@article{osti_1341100,
title = {A flexible microbial co-culture platform for simultaneous utilization of methane and carbon dioxide from gas feedstocks},
author = {Hill, Eric A. and Chrisler, William B. and Beliaev, Alex S. and Bernstein, Hans C.},
abstractNote = {A new co-cultivation technology is presented that converts greenhouse gasses, CH4 and CO2, into microbial biomass. The methanotrophic bacterium, Methylomicrobium alcaliphilum 20z, was coupled to a cyanobacterium, Synechococcus PCC 7002 via oxygenic photosynthesis. The system exhibited robust growth on diverse gas mixtures ranging from biogas to those representative of a natural gas feedstock. A continuous processes was developed on a synthetic natural gas feed that achieved steady-state by imposing coupled light and O2 limitations on the cyanobacterium and methanotroph, respectively. Continuous co-cultivation resulted in an O2 depleted reactor and does not require CH4/O2 mixtures to be fed into the system, thereby enhancing process safety considerations over traditional methanotroph mono-culture platforms. This co-culture technology is scalable with respect to its ability to utilize different gas streams and its biological components constructed from model bacteria that can be metabolically customized to produce a range of biofuels and bioproducts.},
doi = {10.1016/j.biortech.2016.12.111},
journal = {Bioresource Technology},
number = ,
volume = 228,
place = {United States},
year = {Tue Jan 03 00:00:00 EST 2017},
month = {Tue Jan 03 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.biortech.2016.12.111

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