skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles

Abstract

The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. Affinity selection on Nipah Virus-neutralizing monoclonal antibodies using random sequence peptide libraries on MS2 VLPs selected peptides with sequence similarity to peptide sequences found within the envelope glycoprotein of Nipah itself, thus identifying the epitopes the antibodies recognize. The selected peptide sequences themselves are not necessarily identical in all respects to a sequence within Nipah Virus glycoprotein, and therefore may be referred to as epitope mimics VLPs displaying these epitope mimics can serve as vaccine. On the other hand, display of the corresponding wild-type sequence derived from Nipah Virus and corresponding to the epitope mapped by affinity selection, may also be used as a vaccine.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1340588
Patent Number(s):
9,549,976
Application Number:
14/081,629
Assignee:
STC.UNM (Albuquerque, NM) SNL-A
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Nov 15
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Peabody, David S., Chackerian, Bryce, Ashley, Carlee, Carnes, Eric, and Negrete, Oscar. Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles. United States: N. p., 2017. Web.
Peabody, David S., Chackerian, Bryce, Ashley, Carlee, Carnes, Eric, & Negrete, Oscar. Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles. United States.
Peabody, David S., Chackerian, Bryce, Ashley, Carlee, Carnes, Eric, and Negrete, Oscar. Tue . "Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles". United States. https://www.osti.gov/servlets/purl/1340588.
@article{osti_1340588,
title = {Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles},
author = {Peabody, David S. and Chackerian, Bryce and Ashley, Carlee and Carnes, Eric and Negrete, Oscar},
abstractNote = {The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. Affinity selection on Nipah Virus-neutralizing monoclonal antibodies using random sequence peptide libraries on MS2 VLPs selected peptides with sequence similarity to peptide sequences found within the envelope glycoprotein of Nipah itself, thus identifying the epitopes the antibodies recognize. The selected peptide sequences themselves are not necessarily identical in all respects to a sequence within Nipah Virus glycoprotein, and therefore may be referred to as epitope mimics VLPs displaying these epitope mimics can serve as vaccine. On the other hand, display of the corresponding wild-type sequence derived from Nipah Virus and corresponding to the epitope mapped by affinity selection, may also be used as a vaccine.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Peptide Epitope Identification by Affinity Selection on Bacteriophage MS2 Virus-Like Particles
journal, June 2011

  • Chackerian, Bryce; Caldeira, Jerri do Carmo; Peabody, Julianne
  • Journal of Molecular Biology, Vol. 409, Issue 2, p. 225-237
  • DOI: 10.1016/j.jmb.2011.03.072

Subunit Fusion Confers Tolerance to Peptide Insertions in a Virus Coat Protein
journal, November 1997

  • Peabody, David S.
  • Archives of Biochemistry and Biophysics, Vol. 347, Issue 1, p. 85-92
  • DOI: 10.1006/abbi.1997.0312

Immunogenic Display of Diverse Peptides on Virus-like Particles of RNA Phage MS2
journal, June 2008

  • Peabody, David S.; Manifold-Wheeler, Brett; Medford, Alexander
  • Journal of Molecular Biology, Vol. 380, Issue 1, p. 252-263
  • DOI: 10.1016/j.jmb.2008.04.049

Roles of operator and non-operator RNA sequences in bacteriophage R17 capsid assembly
journal, December 1988

  • Beckett, Dorothy; Wu, Huey-Nan; Uhlenbeck, Olke C.
  • Journal of Molecular Biology, Vol. 204, Issue 4, p. 939-947
  • DOI: 10.1016/0022-2836(88)90053-8

BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences
journal, May 1999