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Title: Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source

Journal Article · · Scientific Data
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  1. Uppsala Univ., Uppsala (Sweden)
  2. Uppsala Univ., Uppsala (Sweden); Institute of Physics ASCR, Prague (Czech Republic)
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. European XFEL GmbH, Schenefeld (Germany)
  6. La Trobe Univ., Melbourne (Australia)
  7. SLAC National Accelerator Lab., Menlo Park, CA (United States); Argonne National Lab. (ANL), Argonne, IL (United States); Technische Univ. Berlin, Berlin (Germany)
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  9. SLAC National Accelerator Lab., Menlo Park, CA (United States); Stanford PULSE Institute, Menlo Park, CA (United States)
  10. Cornell Univ., Ithaca, NY (United States)
  11. Arizona State Univ., Tempe, AZ (United States); Biodesign Institute at Arizona State Univ., Tempe, AZ (United States)
  12. Osaka Univ., Osaka (Japan)
  13. Univ. of Wisconsin, Milwaukee, WI (United States)
  14. Gwangju Institute of Science and Technology, Gwangju (Korea)
  15. Beijing Computational Science Research Center, Beijing (China)
  16. National Univ. of Singapore (Singapore)
  17. Pohang Univ. of Science and Technology, Pohang (Korea)
  18. Arizona State Univ., Tempe, AZ (United States)
  19. Uppsala Univ., Uppsala (Sweden); AlbaNova Univ. Center, Stockholm (Sweden)
  20. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); National Research Nuclear Univ. MEPhI (Moscow Engineering Physics Institute), Moscow (Russia)
  21. Brookhaven National Lab. (BNL), Upton, NY (United States)
  22. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
+ Show Author Affiliations

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. Here, the diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Univ. of Wisconsin, Milwaukee, WI (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-76SF00515; SC0002164
OSTI ID:
1340154
Alternate ID(s):
OSTI ID: 1598204
Report Number(s):
SLAC-PUB-16912
Journal Information:
Scientific Data, Vol. 3; ISSN 2052-4463
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 50 works
Citation information provided by
Web of Science

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Coherent diffraction of single Rice Dwarf Virus particles using hard X-rays at the Linac Coherent Light Source (CXIDB ID 36)
  • Munke, Anna
  • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Uppsala University, SLAC National Accelerator Laboratory, ... https://doi.org/10.11577/1252456
dataset January 2016
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Cited By (33)

A statistical approach to detect protein complexes at X-ray free electron laser facilities journal December 2018
The trickle before the torrent—diffraction data from X-ray lasers journal August 2016
Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source journal June 2017
Watching proteins function with time-resolved x-ray crystallography journal August 2017
Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers journal October 2017
Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses journal April 2017
XFELs for structure and dynamics in biology journal May 2017
Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome journal August 2018
Biological single-particle imaging using XFELs – towards the next resolution revolution journal October 2018
Experimental 3D coherent diffractive imaging from photon-sparse random projections journal March 2019
Mask-based approach to phasing of single-particle diffraction data. II. Likelihood-based selection criteria journal January 2019
A guide to sample delivery systems for serial crystallography journal August 2019
Low-signal limit of X-ray single particle diffractive imaging journal January 2019
Single Particle Study by X-Ray Diffraction: Crystallographic Approach journal November 2019
Quantitative ptychographic bio-imaging in the water window text January 2018
Short-wavelength free-electron laser sources and science: a review text January 2017
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene text January 2018
Coherent diffractive imaging of microtubules using an X-ray laser text January 2019
Megahertz single-particle imaging at the European XFEL text January 2020
Determining Complex Structures using Docking Method with Single Particle Scattering Data journal April 2017
The Potential of EuPRAXIA@SPARC_LAB for Radiation Based Techniques journal March 2019
Megahertz single-particle imaging at the European XFEL journal May 2020
Diffraction data of core-shell nanoparticles from an X-ray free electron laser journal April 2017
Single Particle Study by X-Ray Diffraction: Crystallographic Approach journal November 2019
Quantitative ptychographic bio-imaging in the water window journal January 2018
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene journal May 2018
Post-sample aperture for low background diffraction experiments at X-ray free-electron lasers text January 2017
Biological single-particle imaging using XFELs – towards the next resolution revolution text January 2018
Experimental 3D coherent diffractive imaging from photon-sparse random projections text January 2019
Coherent diffractive imaging of microtubules using an X-ray laser journal June 2019
Low-signal limit of X-ray single particle diffractive imaging text January 2019
Short-wavelength free-electron laser sources and science: a review journal October 2017
Single-particle imaging by x-ray free-electron lasers—How many snapshots are needed? journal March 2020

Linked Research (4)

Figures / Tables (8)

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