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Title: Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies

Abstract

Methods for preparing an antibody are provided with the method including incorporating 3-bromo-4-hydroxy-benzoic acid into a protein to form an antigen, immunizing a mammalian host with the antigen, and recovering an antibody having an affinity for the antigen from the host. Antibodies having a binding affinity for a monohalotyrosine are provided as well as composition comprising an antibody bound with monohalotyrosine. Compositions comprising a protein having a 3-bromo-4-hydroxy-benzoic acid moiety are also provided. Methods for evaluating the severity of asthma are provide with the methods including analyzing sputum of a patient using an antibody having a binding affinity for monohalotyrosine, and measuring the amount of antibody bound to protein. Methods for determining eosinophil activity in bodily fluid are also provided with the methods including exposing bodily fluid to an antibody having a binding affinity for monohalotyrosine, and measuring the amount of bound antibody to determine the eosinophil activity.

Inventors:
;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1339636
Patent Number(s):
9,546,220
Application Number:
13/276,608
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC0576RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Oct 19
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Jin, Hongjun, and Zangar, Richard C. Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies. United States: N. p., 2017. Web.
Jin, Hongjun, & Zangar, Richard C. Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies. United States.
Jin, Hongjun, and Zangar, Richard C. Tue . "Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies". United States. https://www.osti.gov/servlets/purl/1339636.
@article{osti_1339636,
title = {Compositions, antibodies, asthma diagnosis methods, and methods for preparing antibodies},
author = {Jin, Hongjun and Zangar, Richard C.},
abstractNote = {Methods for preparing an antibody are provided with the method including incorporating 3-bromo-4-hydroxy-benzoic acid into a protein to form an antigen, immunizing a mammalian host with the antigen, and recovering an antibody having an affinity for the antigen from the host. Antibodies having a binding affinity for a monohalotyrosine are provided as well as composition comprising an antibody bound with monohalotyrosine. Compositions comprising a protein having a 3-bromo-4-hydroxy-benzoic acid moiety are also provided. Methods for evaluating the severity of asthma are provide with the methods including analyzing sputum of a patient using an antibody having a binding affinity for monohalotyrosine, and measuring the amount of antibody bound to protein. Methods for determining eosinophil activity in bodily fluid are also provided with the methods including exposing bodily fluid to an antibody having a binding affinity for monohalotyrosine, and measuring the amount of bound antibody to determine the eosinophil activity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2017},
month = {1}
}

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Works referenced in this record:

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