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Title: Global DNA sequence organization in humans is vertebrate-specific but differs in nuclear and mitochondrial genomes

Abstract

Although genome sequencing projects have made significant progress in the analysis of large amounts of DNA sequence information, little is known regarding primary DNA sequence organization and its determinants. Primary sequence organization was characterized for 15 different regions of the human nuclear genome (each 36 to 180 kb in length and totaling 965 kb), the complete human mitochondrial genome and several viral genomes that infect the human species. Recognition of previously unknown patterns in the organization of large DNA sequences was accomplished using chaos representation and analysis of short-sequence representation. Primary DNA sequence organization was found to have a global structure characterized by a non-random composition and order of nucleotides. Global structure was identified as a higher-order sequence organization that is independent of the functional properties and the length of the DNA sequence. Global sequence organization is similar in the human nuclear genome and viral genomes capable of integration within that genome, supporting the hypothesis of genome-type specific constraints upon primary sequence organization. Human DNA sequence organization was compared with that of four other vertebrate species and four species representing a broad phylogenetic range. Global DNA sequence organization was similar among closely-related species but different in the nuclear and mitochondrialmore » genomes of the same species. Biases in single nucleotide and dinucleotide representation were found to be the major determinants of global structure in mitochondrial and nuclear genomes, respectively. Analysis of DNA sequence evolution must therefore incorporate both gene and genome-type specific mutational and selective constraints.« less

Authors:
;  [1]
  1. Univ. of Western Ontario, London (Canada)
Publication Date:
OSTI Identifier:
133902
Report Number(s):
CONF-941009-
Journal ID: AJHGAG; ISSN 0002-9297; TRN: 95:005313-0636
Resource Type:
Journal Article
Journal Name:
American Journal of Human Genetics
Additional Journal Information:
Journal Volume: 55; Journal Issue: Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN POPULATIONS; AMINO ACID SEQUENCE; VERTEBRATES; MITOCHONDRIA; NUCLEOTIDES; MOLECULAR STRUCTURE; GENES; DNA SEQUENCING; BIOLOGICAL EVOLUTION; GENE MUTATIONS; VIRUSES

Citation Formats

Hill, K A, and Singh, S M. Global DNA sequence organization in humans is vertebrate-specific but differs in nuclear and mitochondrial genomes. United States: N. p., 1994. Web.
Hill, K A, & Singh, S M. Global DNA sequence organization in humans is vertebrate-specific but differs in nuclear and mitochondrial genomes. United States.
Hill, K A, and Singh, S M. 1994. "Global DNA sequence organization in humans is vertebrate-specific but differs in nuclear and mitochondrial genomes". United States.
@article{osti_133902,
title = {Global DNA sequence organization in humans is vertebrate-specific but differs in nuclear and mitochondrial genomes},
author = {Hill, K A and Singh, S M},
abstractNote = {Although genome sequencing projects have made significant progress in the analysis of large amounts of DNA sequence information, little is known regarding primary DNA sequence organization and its determinants. Primary sequence organization was characterized for 15 different regions of the human nuclear genome (each 36 to 180 kb in length and totaling 965 kb), the complete human mitochondrial genome and several viral genomes that infect the human species. Recognition of previously unknown patterns in the organization of large DNA sequences was accomplished using chaos representation and analysis of short-sequence representation. Primary DNA sequence organization was found to have a global structure characterized by a non-random composition and order of nucleotides. Global structure was identified as a higher-order sequence organization that is independent of the functional properties and the length of the DNA sequence. Global sequence organization is similar in the human nuclear genome and viral genomes capable of integration within that genome, supporting the hypothesis of genome-type specific constraints upon primary sequence organization. Human DNA sequence organization was compared with that of four other vertebrate species and four species representing a broad phylogenetic range. Global DNA sequence organization was similar among closely-related species but different in the nuclear and mitochondrial genomes of the same species. Biases in single nucleotide and dinucleotide representation were found to be the major determinants of global structure in mitochondrial and nuclear genomes, respectively. Analysis of DNA sequence evolution must therefore incorporate both gene and genome-type specific mutational and selective constraints.},
doi = {},
url = {https://www.osti.gov/biblio/133902}, journal = {American Journal of Human Genetics},
number = Suppl.3,
volume = 55,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 1994},
month = {Thu Sep 01 00:00:00 EDT 1994}
}