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Title: Identification of potential biomarkers of exposure to diundecyl phthalate

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Journal Article: Publisher's Accepted Manuscript
Journal Name:
Environmental Research
Additional Journal Information:
Journal Volume: 148; Journal Issue: C; Related Information: CHORUS Timestamp: 2017-10-04 15:04:38; Journal ID: ISSN 0013-9351
Country of Publication:
United States

Citation Formats

Silva, Manori J., Bontke, Trevor W., Calafat, Antonia M., and Ye, Xiaoyun. Identification of potential biomarkers of exposure to diundecyl phthalate. United States: N. p., 2016. Web. doi:10.1016/j.envres.2016.03.037.
Silva, Manori J., Bontke, Trevor W., Calafat, Antonia M., & Ye, Xiaoyun. Identification of potential biomarkers of exposure to diundecyl phthalate. United States. doi:10.1016/j.envres.2016.03.037.
Silva, Manori J., Bontke, Trevor W., Calafat, Antonia M., and Ye, Xiaoyun. 2016. "Identification of potential biomarkers of exposure to diundecyl phthalate". United States. doi:10.1016/j.envres.2016.03.037.
title = {Identification of potential biomarkers of exposure to diundecyl phthalate},
author = {Silva, Manori J. and Bontke, Trevor W. and Calafat, Antonia M. and Ye, Xiaoyun},
abstractNote = {},
doi = {10.1016/j.envres.2016.03.037},
journal = {Environmental Research},
number = C,
volume = 148,
place = {United States},
year = 2016,
month = 7

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.envres.2016.03.037

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  • Phthalates are industrial additives widely used as plasticizers. In addition to deleterious effects on male genital development, population studies have documented correlations between phthalates exposure and impacts on reproductive tract development and on the metabolic syndrome in male adults. In this work we investigated potential mechanisms underlying the impact of DEHP on adult mouse liver in vivo. A parallel analysis of hepatic transcript and metabolic profiles from adult mice exposed to varying DEHP doses was performed. Hepatic genes modulated by DEHP are predominantly PPAR{alpha} targets. However, the induction of prototypic cytochrome P450 genes strongly supports the activation of additional NRmore » pathways, including Constitutive Androstane Receptor (CAR). Integration of transcriptomic and metabonomic profiles revealed a correlation between the impacts of DEHP on genes and metabolites related to heme synthesis and to the Rev-erb{alpha} pathway that senses endogenous heme level. We further confirmed the combined impact of DEHP on the hepatic expression of Alas1, a critical enzyme in heme synthesis and on the expression of Rev-erb{alpha} target genes involved in the cellular clock and in energy metabolism. This work shows that DEHP interferes with hepatic CAR and Rev-erb{alpha} pathways which are both involved in the control of metabolism. The identification of these new hepatic pathways targeted by DEHP could contribute to metabolic and endocrine disruption associated with phthalate exposure. Gene expression profiles performed on microdissected testis territories displayed a differential responsiveness to DEHP. Altogether, this suggests that impacts of DEHP on adult organs, including testis, could be documented and deserve further investigations.« less
  • The radiation chemistry of poly(vinyl chloride) (PVC) blended with trimethylolpropanetrimethacrylate (TMPTMA) and diundecyl phthalate (DUP) has been examined. This three-component mixture contains a base resin (PVC), a crosslinking sensitizer (TMPTMA), and a physical modifier (DUP). These are the basic components in any radiation-curable coating. The kinetics and mechanism of the crosslinking reactions were studied with reference to the dependence on radiation dose and blend composition. The polyfunctional TMPTMA underwent polymerization incorporating the PVC into a 3-dimensional network. DUP remained chemically inert during the irradiation, not being bound to the network. However, DUP by plasticizing the macromolecules and diluting the monomer,more » changed the kinetics extensively. DUP enhanced TMPTMA homopolymerization, TMPTMA grafting, and PVC crosslinking reaction rates. The effect of the competition between polymerization, grafting, and degradation reactions was examined in terms of enhanced mobility of the reacting species. The influence of these kinetics considerations in selecting a blend composition for a coating application was discussed.« less
  • Blends of poly(vinyl chloride)(PVC) with polyfunctional monomers may be crosslinked by ionizing radiation. The physical properties of PVC blended with trimethylolpropanetrimethacrylate (TMPTMA) and diundecyl phthalate (DUP) were studied. The TMPTMA monomer crosslinked the blend by homopolymerization and/or grafting to PVC. The plasticizer, DUP, was chemically inert under irradiation but, by plasticizing the macromolecules and diluting the monomer, changed the kinetics extensively. Characteristics of the glass transitions and the tensile mechanical properties have been correlated with blend composition and radiation dose. Before irradiation, poly(vinyl chloride) was plasticized by both DUP and TMPTMA monomer. The increase in glass transition temperature and mechanicalmore » strength following irradiation to 5 Mrad was correlated with the TMPTMA content of the blend. Both the molecular structure of the network and the DUP content of the blend were factors in determining the physical properties of the final crosslinked blend. The molecular structure was determined by the kinetics of the crosslinking reactions, which in turn were determined by the blend composition. A molecular interpretation consistent with the physical properties, chemical kinetics, and mechanism of the crosslinking system has been presented. 24 figures, 2 tables.« less
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