Preserving elemental content in adherent mammalian cells for analysis by synchrotron-based x-ray fluorescence microscopy

JIN, QIAOLING; PAUNESKU, TATJANA; LAI, BARRY; GLEBER, SOPHIE-CHARLOTTE; CHEN, SI; FINNEY, LYDIA; VINE, DAVID; VOGT, STEFAN; WOLOSCHAK, GAYLE; JACOBSEN, CHRIS

doi:10.1111/jmi.12466

Title: Preserving elemental content in adherent mammalian cells for analysis by synchrotron-based x-ray fluorescence microscopy

Journal Article · Wed Aug 31 00:00:00 EDT 2016 · Journal of Microscopy

DOI:https://doi.org/10.1111/jmi.12466· OSTI ID:1336508

JIN, QIAOLING ^[1]; PAUNESKU, TATJANA ^[2]; LAI, BARRY ^[3]; GLEBER, SOPHIE-CHARLOTTE ^[3]; CHEN, SI ^[3]; FINNEY, LYDIA ^[3]; VINE, DAVID ^[3]; VOGT, STEFAN ^[3]; WOLOSCHAK, GAYLE ^[2]; JACOBSEN, CHRIS ^[4]

Department of Physics & Astronomy, Weinberg College of Arts and Sciences, Evanston Illinois U.S.A
Department of Radiation Oncology, Northwestern University, Chicago Illinois U.S.A
Advanced Photon Source, Argonne National Laboratory, Argonne Illinois U.S.A
Department of Physics & Astronomy, Weinberg College of Arts and Sciences, Evanston Illinois U.S.A, Advanced Photon Source, Argonne National Laboratory, Argonne Illinois U.S.A

Summary Trace metals play important roles in biological function, and x‐ray fluorescence microscopy (XFM) provides a way to quantitatively image their distribution within cells. The faithfulness of these measurements is dependent on proper sample preparation. Using mouse embryonic fibroblast NIH/3T3 cells as an example, we compare various approaches to the preparation of adherent mammalian cells for XFM imaging under ambient temperature. Direct side‐by‐side comparison shows that plunge‐freezing‐based cryoimmobilization provides more faithful preservation than conventional chemical fixation for most biologically important elements including P, S, Cl, K, Fe, Cu, Zn and possibly Ca in adherent mammalian cells. Although cells rinsed with fresh media had a great deal of extracellular background signal for Cl and Ca, this approach maintained cells at the best possible physiological status before rapid freezing and it does not interfere with XFM analysis of other elements. If chemical fixation has to be chosen, the combination of 3% paraformaldehyde and 1.5 % glutaraldehyde preserves S, Fe, Cu and Zn better than either fixative alone. When chemically fixed cells were subjected to a variety of dehydration processes, air drying was proved to be more suitable than other drying methods such as graded ethanol dehydration and freeze drying. This first detailed comparison for x‐ray fluorescence microscopy shows how detailed quantitative conclusions can be affected by the choice of cell preparation method.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357; R01 GM104530; DE‐AC02‐06CH11357

OSTI ID:: 1336508

Alternate ID(s):: OSTI ID: 1390261; OSTI ID: 1464537

Journal Information:: Journal of Microscopy, Journal Name: Journal of Microscopy Vol. 265 Journal Issue: 1; ISSN 0022-2720

Publisher:: WileyCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 54 works

Citation information provided by
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