skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Dietary Polyunsaturated Fatty Acids Increase Survival and Decrease Bacterial Load during Septic Staphylococcus aureus Infection and Improve Neutrophil Function in Mice

Abstract

Severe infection, including sepsis, is an increasing clinical problem that causes prolonged morbidity and substantial mortality. At present, antibiotics are essentially the only pharmacological treatment for sepsis. Furthermore, the incidence of resistance to antibiotics is increasing; therefore, it is critical to find new therapies for sepsis.Staphylococcus aureusis a major cause of septic mortality. Neutrophils play an important role in the defense against bacterial infections. We have shown that a diet with high levels of dietary saturated fatty acids decreases survival in septic mice, but the mechanisms behind this remain elusive. The aim of the present study was to investigate how the differences in dietary fat composition affect survival and bacterial load after experimental septic infection and neutrophil function in uninfected mice. We also found that, afterS. aureusinfection, mice fed a polyunsaturated high-fat diet (HFD-P) for 8 weeks had increased survival and decreased bacterial load during sepsis compared with mice fed a saturated high-fat diet (HFD-S), similar to mice fed a low-fat diet (LFD). Uninfected mice fed HFD-P had a higher frequency of neutrophils in bone marrow than mice fed HFD-S. In addition, mice fed HFD-P had a higher frequency of neutrophils recruited to the site of inflammation in response tomore » peritoneal injection of thioglycolate than mice fed HFD-S. Differences between the proportion of dietary protein and carbohydrate did not affect septic survival at all. Finally, polyunsaturated dietary fat increased both survival and efficiency of bacterial clearance during septicS. aureusinfection. Moreover, this diet increased the frequency and chemotaxis of neutrophils, key components of the immune response toS. aureusinfections.« less

Authors:
ORCiD logo [1];  [2];  [1];  [3];  [1];  [4];  [1];  [1];  [1];  [5];  [1];  [1];
  1. Univ. of Gothenburg (Sweden). Inst. of Neuroscience and Physiology
  2. Univ. of Gothenburg (Sweden). Sahlgrenska Academy
  3. Chalmers Univ. of Technology, Gothenburg (Sweden). Dept. of Chemical and Biological Engineering and Dept. of Mathematical Statistics
  4. Chalmers Univ. of Technology, Gothenburg (Sweden). Dept. of Mathematical Statistics
  5. Chalmers Univ. of Technology, Gothenburg (Sweden).
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1334439
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Infection and Immunity
Additional Journal Information:
Journal Volume: 83; Journal Issue: 2; Journal ID: ISSN 0019-9567
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Svahn, Sara L., Grahnemo, Louise, Pálsdóttir, Vilborg, Nookaew, Intawat, Wendt, Karl, Gabrielsson, Britt, Schéle, Erik, Benrick, Anna, Andersson, Niklas, Nilsson, Staffan, Johansson, Maria E., Jansson, John-Olov, and McCormick, B. A. Dietary Polyunsaturated Fatty Acids Increase Survival and Decrease Bacterial Load during Septic Staphylococcus aureus Infection and Improve Neutrophil Function in Mice. United States: N. p., 2014. Web. doi:10.1128/IAI.02349-14.
Svahn, Sara L., Grahnemo, Louise, Pálsdóttir, Vilborg, Nookaew, Intawat, Wendt, Karl, Gabrielsson, Britt, Schéle, Erik, Benrick, Anna, Andersson, Niklas, Nilsson, Staffan, Johansson, Maria E., Jansson, John-Olov, & McCormick, B. A. Dietary Polyunsaturated Fatty Acids Increase Survival and Decrease Bacterial Load during Septic Staphylococcus aureus Infection and Improve Neutrophil Function in Mice. United States. doi:10.1128/IAI.02349-14.
Svahn, Sara L., Grahnemo, Louise, Pálsdóttir, Vilborg, Nookaew, Intawat, Wendt, Karl, Gabrielsson, Britt, Schéle, Erik, Benrick, Anna, Andersson, Niklas, Nilsson, Staffan, Johansson, Maria E., Jansson, John-Olov, and McCormick, B. A. Mon . "Dietary Polyunsaturated Fatty Acids Increase Survival and Decrease Bacterial Load during Septic Staphylococcus aureus Infection and Improve Neutrophil Function in Mice". United States. doi:10.1128/IAI.02349-14.
@article{osti_1334439,
title = {Dietary Polyunsaturated Fatty Acids Increase Survival and Decrease Bacterial Load during Septic Staphylococcus aureus Infection and Improve Neutrophil Function in Mice},
author = {Svahn, Sara L. and Grahnemo, Louise and Pálsdóttir, Vilborg and Nookaew, Intawat and Wendt, Karl and Gabrielsson, Britt and Schéle, Erik and Benrick, Anna and Andersson, Niklas and Nilsson, Staffan and Johansson, Maria E. and Jansson, John-Olov and McCormick, B. A.},
abstractNote = {Severe infection, including sepsis, is an increasing clinical problem that causes prolonged morbidity and substantial mortality. At present, antibiotics are essentially the only pharmacological treatment for sepsis. Furthermore, the incidence of resistance to antibiotics is increasing; therefore, it is critical to find new therapies for sepsis.Staphylococcus aureusis a major cause of septic mortality. Neutrophils play an important role in the defense against bacterial infections. We have shown that a diet with high levels of dietary saturated fatty acids decreases survival in septic mice, but the mechanisms behind this remain elusive. The aim of the present study was to investigate how the differences in dietary fat composition affect survival and bacterial load after experimental septic infection and neutrophil function in uninfected mice. We also found that, afterS. aureusinfection, mice fed a polyunsaturated high-fat diet (HFD-P) for 8 weeks had increased survival and decreased bacterial load during sepsis compared with mice fed a saturated high-fat diet (HFD-S), similar to mice fed a low-fat diet (LFD). Uninfected mice fed HFD-P had a higher frequency of neutrophils in bone marrow than mice fed HFD-S. In addition, mice fed HFD-P had a higher frequency of neutrophils recruited to the site of inflammation in response to peritoneal injection of thioglycolate than mice fed HFD-S. Differences between the proportion of dietary protein and carbohydrate did not affect septic survival at all. Finally, polyunsaturated dietary fat increased both survival and efficiency of bacterial clearance during septicS. aureusinfection. Moreover, this diet increased the frequency and chemotaxis of neutrophils, key components of the immune response toS. aureusinfections.},
doi = {10.1128/IAI.02349-14},
journal = {Infection and Immunity},
issn = {0019-9567},
number = 2,
volume = 83,
place = {United States},
year = {2014},
month = {11}
}

Works referenced in this record:

Antibacterial resistance worldwide: causes, challenges and responses
journal, November 2004

  • Levy, Stuart B.; Marshall, Bonnie
  • Nature Medicine, Vol. 10, Issue S12
  • DOI: 10.1038/nm1145

Mouse blood monocytes: Standardizing their identification and analysis using CD115
journal, April 2013

  • Breslin, W. L.; Strohacker, K.; Carpenter, K. C.
  • Journal of Immunological Methods, Vol. 390, Issue 1-2
  • DOI: 10.1016/j.jim.2011.03.005

Regulation of Hematopoietic Stem Cells by the Steel Factor/KIT Signaling Pathway
journal, April 2008


Role of glutamine in immunologic responses
journal, July 1998


Regulation of neutrophil trafficking from the bone marrow
journal, November 2011


Caspase-8 is activated by cathepsin D initiating neutrophil apoptosis during the resolution of inflammation
journal, February 2008

  • Conus, Sébastien; Perozzo, Remo; Reinheckel, Thomas
  • The Journal of Experimental Medicine, Vol. 205, Issue 3
  • DOI: 10.1084/jem.20072152

A Cardinal Role for Cathepsin D in Co-Ordinating the Host-Mediated Apoptosis of Macrophages and Killing of Pneumococci
journal, January 2011


Toward an Epidemiology and Natural History of SIRS (Systemic Inflammatory Response Syndrome)
journal, December 1992


Neutrophils in innate host defense against Staphylococcus aureus infections
journal, November 2011



journal, January 2003


Controlling the false discovery rate in behavior genetics research
journal, November 2001


Use of within-array replicate spots for assessing differential expression in microarray experiments
journal, January 2005


The Pathophysiology and Treatment of Sepsis
journal, January 2003

  • Hotchkiss, Richard S.; Karl, Irene E.
  • New England Journal of Medicine, Vol. 348, Issue 2
  • DOI: 10.1056/NEJMra021333

Neutrophils and immunity: challenges and opportunities
journal, February 2006

  • Nathan, Carl
  • Nature Reviews Immunology, Vol. 6, Issue 3
  • DOI: 10.1038/nri1785

Animal Models of Sepsis and Shock: a Review and Lessons Learned
journal, January 1998


Types of Dietary Fat and Risk of Coronary Heart Disease: A Critical Review
journal, February 2001

  • Hu, Frank B.; Manson, JoAnn E.; Willett, Walter C.
  • Journal of the American College of Nutrition, Vol. 20, Issue 1
  • DOI: 10.1080/07315724.2001.10719008

Microinjection of Cathepsin D Induces Caspase-Dependent Apoptosis in Fibroblasts
journal, July 2002


Emerging drugs for the treatment of sepsis
journal, July 2012

  • Kotsaki, Antigone; Giamarellos-Bourboulis, Evangelos J.
  • Expert Opinion on Emerging Drugs, Vol. 17, Issue 3
  • DOI: 10.1517/14728214.2012.697151

Cutting Edge: A Novel Nonoxidative Phagosomal Mechanism Exerted by Cathepsin-D Controls Listeria monocytogenes Intracellular Growth
journal, January 2006

  • Cerro-Vadillo, Elida del; Madrazo-Toca, Fidel; Carrasco-Marín, Eugenio
  • The Journal of Immunology, Vol. 176, Issue 3
  • DOI: 10.4049/jimmunol.176.3.1321

Effect of Glutamine on Phagocytosis and Bacterial Killing by Normal and Pediatric Burn Patient Neutrophils
journal, March 1994

  • Ogle, Cora K.; Ogle, James D.; Mao, Ju-Xian
  • Journal of Parenteral and Enteral Nutrition, Vol. 18, Issue 2
  • DOI: 10.1177/0148607194018002128

Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
journal, February 1992

  • Ikuta, K.; Weissman, I. L.
  • Proceedings of the National Academy of Sciences, Vol. 89, Issue 4
  • DOI: 10.1073/pnas.89.4.1502

2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference
journal, January 2003


The neutrophil in vascular inflammation
journal, November 2011

  • Phillipson, Mia; Kubes, Paul
  • Nature Medicine, Vol. 17, Issue 11
  • DOI: 10.1038/nm.2514

Effect of a Glutamine-Supplemented Diet on Response to Methicillin-Resistant Staphylococcus aureus Infection in Mice.
journal, January 1993

  • Suzuki, Isao; Matsumoto, Yoko; Adjei, Andrew A.
  • Journal of Nutritional Science and Vitaminology, Vol. 39, Issue 4
  • DOI: 10.3177/jnsv.39.405

The Epidemiology of Sepsis in the United States from 1979 through 2000
journal, April 2003

  • Martin, Greg S.; Mannino, David M.; Eaton, Stephanie
  • New England Journal of Medicine, Vol. 348, Issue 16
  • DOI: 10.1056/NEJMoa022139

Management of Sepsis
journal, October 2006


Mice Chronically Fed High-Fat Diet Have Increased Mortality and Disturbed Immune Response in Sepsis
journal, October 2009


Inhibition of CCL20 increases mortality in models of mouse sepsis with intestinal apoptosis
journal, July 2013


Immunity against Staphylococcus aureus cutaneous infections
journal, July 2011

  • Miller, Lloyd S.; Cho, John S.
  • Nature Reviews Immunology, Vol. 11, Issue 8
  • DOI: 10.1038/nri3010