Rapid, portable, multiplexed detection of bacterial pathogens directly from clinical sample matrices

Phaneuf, Christopher R.; Mangadu, Betty Lou Bosano; Piccini, Matthew E.; Singh, Anup K.; Koh, Chung -Yan

doi:10.3390/bios6040049

Title: Rapid, portable, multiplexed detection of bacterial pathogens directly from clinical sample matrices

Journal Article · Fri Sep 23 00:00:00 EDT 2016 · Biosensors

DOI:https://doi.org/10.3390/bios6040049· OSTI ID:1333619

Phaneuf, Christopher R. ^[1]; Mangadu, Betty Lou Bosano ^[1]; Piccini, Matthew E. ^[2]; Singh, Anup K. ^[1]; Koh, Chung -Yan ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Cepheid, Sunnyvale, CA (United States)

Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. Furthermore, this platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min.

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Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1333619

Report Number(s):: SAND-2016-8183J; BIOSHU; 646818

Journal Information:: Biosensors, Vol. 6, Issue 4; ISSN 2079-6374

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

References (34)

Molecular Diagnosis of Diarrhea: Current Status and Future Potential Platts-Mills, James A.; Operario, Darwin J.; Houpt, Eric R. Current Infectious Disease Reports, Vol. 14, Issue 1 https://doi.org/10.1007/s11908-011-0223-7	journal	November 2011
Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples Vaisocherová-Lísalová, Hana; Víšová, Ivana; Ermini, Maria Laura Biosensors and Bioelectronics, Vol. 80 https://doi.org/10.1016/j.bios.2016.01.040	journal	June 2016
Centrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin Koh, Chung-Yan; Schaff, Ulrich Y.; Piccini, Matthew E. Analytical Chemistry, Vol. 87, Issue 2, p. 922-928 https://doi.org/10.1021/ac504054u	journal	December 2014
A centrifugal fluidic immunoassay for ocular diagnostics with an enzymatically hydrolyzed fluorogenic substrate Walsh III, David I.; Sommer, Gregory J.; Schaff, Ulrich Y. Lab on a Chip, Vol. 14, Issue 15 https://doi.org/10.1039/c4lc00279b	journal	January 2014
Efficient and Rapid Detection of Salmonella Using Microfluidic Impedance Based Sensing Ghosh Dastider, Shibajyoti; Barizuddin, Syed; Yuksek, Nuh S. Journal of Sensors, Vol. 2015 https://doi.org/10.1155/2015/293461	journal	January 2015
Challenges in the Use of Compact Disc-Based Centrifugal Microfluidics for Healthcare Diagnostics at the Extreme Point of Care Gilmore, Jordon; Islam, Monsur; Martinez-Duarte, Rodrigo Micromachines, Vol. 7, Issue 4 https://doi.org/10.3390/mi7040052	journal	March 2016
Setting Research Priorities to Reduce Mortality and Morbidity of Childhood Diarrhoeal Disease in the Next 15 Years Wazny, Kerri; Zipursky, Alvin; Black, Robert PLoS Medicine, Vol. 10, Issue 5 https://doi.org/10.1371/journal.pmed.1001446	journal	May 2013
Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples Bunyakul, Natinan; Baeumner, Antje Sensors, Vol. 15, Issue 1 https://doi.org/10.3390/s150100547	journal	December 2014
Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation Schaff, U. Y.; Sommer, G. J. Clinical Chemistry, Vol. 57, Issue 5, p. 753-761 https://doi.org/10.1373/clinchem.2011.162206	journal	March 2011
CD-Based Microfluidics for Primary Care in Extreme Point-of-Care Settings Smith, Suzanne; Mager, Dario; Perebikovsky, Alexandra Micromachines, Vol. 7, Issue 2 https://doi.org/10.3390/mi7020022	journal	January 2016
Identifying multiple bacterial pathogens by loop-mediated isothermal amplification on a rotate & react slipchip Xia, Yun; Liu, Zhihua; Yan, Shuangqian Sensors and Actuators B: Chemical, Vol. 228 https://doi.org/10.1016/j.snb.2016.01.074	journal	June 2016
A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection Sayad, Abkar Ahmed; Ibrahim, Fatimah; Uddin, Shah Mukim Sensors and Actuators B: Chemical, Vol. 227 https://doi.org/10.1016/j.snb.2015.10.116	journal	May 2016
A Review of Biomedical Centrifugal Microfluidic Platforms Tang, Minghui; Wang, Guanghui; Kong, Siu-Kai Micromachines, Vol. 7, Issue 2 https://doi.org/10.3390/mi7020026	journal	February 2016
Real-time loop-mediated isothermal DNA amplification in compact disc micro-reactors Santiago-Felipe, Sara; Tortajada-Genaro, Luis A.; Carrascosa, Javier Biosensors and Bioelectronics, Vol. 79 https://doi.org/10.1016/j.bios.2015.12.045	journal	May 2016
An integrated direct loop-mediated isothermal amplification microdevice incorporated with an immunochromatographic strip for bacteria detection in human whole blood and milk without a sample preparation step Lee, Dohwan; Kim, Yong Tae; Lee, Jee Won Biosensors and Bioelectronics, Vol. 79 https://doi.org/10.1016/j.bios.2015.12.044	journal	May 2016
Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water Litvinov, Julia; Moen, Scott T.; Koh, Chung-Yan Biomicrofluidics, Vol. 10, Issue 1 https://doi.org/10.1063/1.4939099	journal	January 2016
Global Causes of Diarrheal Disease Mortality in Children <5 Years of Age: A Systematic Review Lanata, Claudio F.; Fischer-Walker, Christa L.; Olascoaga, Ana C. PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0072788	journal	September 2013
Centrifugal microfluidics for biomedical applications Gorkin, Robert; Park, Jiwoon; Siegrist, Jonathan Lab on a Chip, Vol. 10, Issue 14, p. 1758-1773 https://doi.org/10.1039/b924109d	journal	January 2010
Effect of Macromolecular Crowding on Protein Binding Stability: Modest Stabilization and Significant Biological Consequences Batra, Jyotica; Xu, Ke; Qin, Sanbo Biophysical Journal, Vol. 97, Issue 3 https://doi.org/10.1016/j.bpj.2009.05.032	journal	August 2009
Microfluidic diagnostic technologies for global public health Yager, Paul; Edwards, Thayne; Fu, Elain Nature, Vol. 442, Issue 7101, p. 412-418 https://doi.org/10.1038/nature05064	journal	July 2006
Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving Amasia, Mary; Cozzens, Meghan; Madou, Marc J. Sensors and Actuators B: Chemical, Vol. 161, Issue 1 https://doi.org/10.1016/j.snb.2011.11.080	journal	January 2012
Macromolecular crowding effects on protein–protein binding affinity and specificity Kim, Young C.; Best, Robert B.; Mittal, Jeetain The Journal of Chemical Physics, Vol. 133, Issue 20 https://doi.org/10.1063/1.3516589	journal	November 2010
Advances and challenges in biosensor-based diagnosis of infectious diseases Sin, Mandy LY; Mach, Kathleen E.; Wong, Pak Kin Expert Review of Molecular Diagnostics, Vol. 14, Issue 2 https://doi.org/10.1586/14737159.2014.888313	journal	February 2014
Centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection Oh, Seung Jun; Park, Byung Hyun; Jung, Jae Hwan Biosensors and Bioelectronics, Vol. 75 https://doi.org/10.1016/j.bios.2015.08.052	journal	January 2016
Where and why are 10 million children dying every year? Black, Robert E.; Morris, Saul S.; Bryce, Jennifer The Lancet, Vol. 361, Issue 9376 https://doi.org/10.1016/S0140-6736(03)13779-8	journal	June 2003
Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000 Liu, Li; Johnson, Hope L.; Cousens, Simon The Lancet, Vol. 379, Issue 9832 https://doi.org/10.1016/S0140-6736(12)60560-1	journal	June 2012
Diarrhoea: why children are still dying and what can be done Wardlaw, Tessa; Salama, Peter; Brocklehurst, Clarissa The Lancet, Vol. 375, Issue 9718 https://doi.org/10.1016/S0140-6736(09)61798-0	journal	March 2010
Multicenter Evaluation of the BD Max Enteric Bacterial Panel PCR Assay for Rapid Detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga Toxin 1 and 2 Genes Harrington, S. M.; Buchan, B. W.; Doern, C. Journal of Clinical Microbiology, Vol. 53, Issue 5 https://doi.org/10.1128/JCM.03480-14	journal	March 2015
Rapid and fully automated bacterial pathogen detection on a centrifugal-microfluidic LabDisk using highly sensitive nested PCR with integrated sample preparation Czilwik, G.; Messinger, T.; Strohmeier, O. Lab on a Chip, Vol. 15, Issue 18 https://doi.org/10.1039/C5LC00591D	journal	January 2015
Fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device Oh, Seung Jun; Park, Byung Hyun; Choi, Goro Lab on a Chip, Vol. 16, Issue 10 https://doi.org/10.1039/C6LC00326E	journal	January 2016
Centrifugal microfluidic platforms: advanced unit operations and applications Strohmeier, O.; Keller, M.; Schwemmer, F. Chemical Society Reviews, Vol. 44, Issue 17 https://doi.org/10.1039/C4CS00371C	journal	January 2015
Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples Bunyakul, N.; Bäumner, Antje J. Universität Regensburg https://doi.org/10.5283/epub.41657	text	January 2015
CD-based microfluidics for primary care in extreme point-of-care settings Smith, S.; Mager, D.; Perebikovsky, A. Karlsruhe https://doi.org/10.5445/ir/1000053234	text	January 2016
Global regional and national causes of child mortality Modell, Bernadette; Berry, Rj; Boyle, Coleen A. The Lancet, Vol. 380, Issue 9853 https://doi.org/10.1016/s0140-6736(12)61878-9	journal	November 2012

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