skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater

Abstract

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive released to the environment as a result of weapons manufacturing and testing worldwide. At Los Alamos National Laboratory, the Technical Area (TA) 16 260 Outfall discharged high-explosives-bearing water from a high-explosives-machining facility to Cañon de Valle during 1951 through 1996. These discharges served as a primary source of high-explosives and inorganic-element contamination in the area. Data indicate that springs, surface water, alluvial groundwater, and perched-intermediate groundwater contain explosive compounds, including RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine); HMX (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine); and TNT (2,4,6-trinitrotoluene). RDX has been detected in the regional aquifer in several wells, and a corrective measures evaluation is planned to identify remedial alternatives to protect the regional aquifer. Perched-intermediate groundwater at Technical Area 16 is present at depths from 650 ft to 1200 ft bgs. In this study, we examined the microbial diversity in a monitoring well completed in perched-intermediate groundwater contaminated by RDX, and examined the response of the microbial population to biostimulation under varying geochemical conditions. Results show that the groundwater microbiome was dominated by Actinobacteria and Proteobacteria. A total of 1,605 operational taxonomic units (OTUs) in 96 bacterial genera were identified. Rhodococcus was the most abundant genus (30.6%) and a total of 46 OTUs weremore » annotated as Rhodococcus. One OTU comprising 25.2% of total sequences was closely related to a RDX -degrading strain R. erythropolis HS4. A less abundant OTU from the Pseudomonas family closely related to RDX-degrading strain P. putida II-B was also present. Biostimulation significantly enriched Proteobacteria but decreased/eliminated the population of Actinobacteria. Consistent with RDX degradation, the OTU closely related to the RDX-degrading P. putida strain II-B was specifically enriched in the RDX-degrading samples. Analysis of the accumulation of RDX-degradation products reveals that during active RDX degradation, there is a transient increase in the concentration of the degradation products MNX, DNX, TNX, and NDAB. The accumulation of these degradation products suggests that RDX is degraded via sequential reduction of the nitro functional groups followed by abiotic ring-cleavage. Here, the results suggest that strict anaerobic conditions are needed to stimulate RDX degradation under the TA-16 site-specific conditions.« less

Authors:
 [1];  [1];  [1];  [1];  [2];  [3];  [4];  [4];  [4];  [4]
  1. Earth Systems Observations EES-14, Earth and Environmental Sciences Division, Los Alamos National Laboratory, Los Alamos NM USA
  2. Environmental Programs ADEP, Los Alamos National Laboratory, Los Alamos NM USA
  3. School of Science and Technology, Georgia Gwinnett College, Lawrenceville GA USA
  4. Bioenergy and Biome Sciences, Biology Sciences Division, Los Alamos National Laboratory, Los Alamos NM USA
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Environmental Management (EM)
OSTI Identifier:
1332767
Alternate Identifier(s):
OSTI ID: 1333023; OSTI ID: 1338778
Report Number(s):
LA-UR-16-23502
Journal ID: ISSN 2045-8827
Grant/Contract Number:  
AC52-06NA25396
Resource Type:
Journal Article: Published Article
Journal Name:
MicrobiologyOpen
Additional Journal Information:
Journal Volume: 6; Journal Issue: 2; Journal ID: ISSN 2045-8827
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biological Science; Earth Sciences; Environmental Protection; Biostimulation, hexahydro-1,3,5-trinitro-1,3,5-triazine, bacterial profiling

Citation Formats

Wang, Dongping, Boukhalfa, Hakim, Marina, Oana, Ware, Doug S., Goering, Tim J., Sun, Fengjie, Daligault, Hajnalka E., Lo, Chien-Chi, Vuyisich, Momchilo, and Starkenburg, Shawn R. Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater. United States: N. p., 2016. Web. doi:10.1002/mbo3.423.
Wang, Dongping, Boukhalfa, Hakim, Marina, Oana, Ware, Doug S., Goering, Tim J., Sun, Fengjie, Daligault, Hajnalka E., Lo, Chien-Chi, Vuyisich, Momchilo, & Starkenburg, Shawn R. Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater. United States. doi:10.1002/mbo3.423.
Wang, Dongping, Boukhalfa, Hakim, Marina, Oana, Ware, Doug S., Goering, Tim J., Sun, Fengjie, Daligault, Hajnalka E., Lo, Chien-Chi, Vuyisich, Momchilo, and Starkenburg, Shawn R. Thu . "Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater". United States. doi:10.1002/mbo3.423.
@article{osti_1332767,
title = {Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater},
author = {Wang, Dongping and Boukhalfa, Hakim and Marina, Oana and Ware, Doug S. and Goering, Tim J. and Sun, Fengjie and Daligault, Hajnalka E. and Lo, Chien-Chi and Vuyisich, Momchilo and Starkenburg, Shawn R.},
abstractNote = {Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is a high explosive released to the environment as a result of weapons manufacturing and testing worldwide. At Los Alamos National Laboratory, the Technical Area (TA) 16 260 Outfall discharged high-explosives-bearing water from a high-explosives-machining facility to Cañon de Valle during 1951 through 1996. These discharges served as a primary source of high-explosives and inorganic-element contamination in the area. Data indicate that springs, surface water, alluvial groundwater, and perched-intermediate groundwater contain explosive compounds, including RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine); HMX (octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine); and TNT (2,4,6-trinitrotoluene). RDX has been detected in the regional aquifer in several wells, and a corrective measures evaluation is planned to identify remedial alternatives to protect the regional aquifer. Perched-intermediate groundwater at Technical Area 16 is present at depths from 650 ft to 1200 ft bgs. In this study, we examined the microbial diversity in a monitoring well completed in perched-intermediate groundwater contaminated by RDX, and examined the response of the microbial population to biostimulation under varying geochemical conditions. Results show that the groundwater microbiome was dominated by Actinobacteria and Proteobacteria. A total of 1,605 operational taxonomic units (OTUs) in 96 bacterial genera were identified. Rhodococcus was the most abundant genus (30.6%) and a total of 46 OTUs were annotated as Rhodococcus. One OTU comprising 25.2% of total sequences was closely related to a RDX -degrading strain R. erythropolis HS4. A less abundant OTU from the Pseudomonas family closely related to RDX-degrading strain P. putida II-B was also present. Biostimulation significantly enriched Proteobacteria but decreased/eliminated the population of Actinobacteria. Consistent with RDX degradation, the OTU closely related to the RDX-degrading P. putida strain II-B was specifically enriched in the RDX-degrading samples. Analysis of the accumulation of RDX-degradation products reveals that during active RDX degradation, there is a transient increase in the concentration of the degradation products MNX, DNX, TNX, and NDAB. The accumulation of these degradation products suggests that RDX is degraded via sequential reduction of the nitro functional groups followed by abiotic ring-cleavage. Here, the results suggest that strict anaerobic conditions are needed to stimulate RDX degradation under the TA-16 site-specific conditions.},
doi = {10.1002/mbo3.423},
journal = {MicrobiologyOpen},
number = 2,
volume = 6,
place = {United States},
year = {Thu Nov 17 00:00:00 EST 2016},
month = {Thu Nov 17 00:00:00 EST 2016}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1002/mbo3.423

Save / Share:

Works referenced in this record:

Confidence Limits on Phylogenies: An Approach Using the Bootstrap
journal, July 1985

  • Felsenstein, Joseph
  • Evolution, Vol. 39, Issue 4, p. 783-791
  • DOI: 10.2307/2408678