A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

Rabe, Franziska; Bosch, Jason; Stirnberg, Alexandra; Guse, Tilo; Bauer, Lisa; Seitner, Denise; Rabanal, Fernando A.; Czedik-Eysenberg, Angelika; Uhse, Simon; Bindics, Janos; Genenncher, Bianca; Navarrete, Fernando; Kellner, Ronny; Ekker, Heinz; Kumlehn, Jochen; Vogel, John P.; Gordon, Sean P.; Marcel, Thierry C.; Münsterkötter, Martin; Walter, Mathias C.; Sieber, Christian MK; Mannhaupt, Gertrud; Güldener, Ulrich; Kahmann, Regine; Djamei, Armin

doi:10.7554/eLife.20522

Title: A complete toolset for the study of Ustilago bromivora and Brachypodium sp. as a fungal-temperate grass pathosystem

Journal Article · Fri Nov 11 00:00:00 EST 2016 · eLife

DOI:https://doi.org/10.7554/eLife.20522· OSTI ID:1331828

Rabe, Franziska ^[1]; Bosch, Jason ^[2]; Stirnberg, Alexandra ^[2]; Guse, Tilo ^[2]; Bauer, Lisa ^[2]; Seitner, Denise ^[2]; Rabanal, Fernando A. ^[2]; Czedik-Eysenberg, Angelika ^[2]; Uhse, Simon ^[2]; Bindics, Janos ^[2]; Genenncher, Bianca ^[2]; Navarrete, Fernando ^[2];

^[3]; Ekker, Heinz ^[4]; Kumlehn, Jochen ^[5]; Vogel, John P. ^[6]; Gordon, Sean P. ^[6]; Marcel, Thierry C. ^[7]; Münsterkötter, Martin ^[8];

^[9] more »

Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany
Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, Austria
Max Planck Institute for Plant Breeding Research, Cologne, Germany
Vienna Biocenter Core Facilities GmbH, Vienna, Austria
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany
DOE Joint Genome Institute, California, United States
INRA UMR BIOGER, AgroParisTech, Université Paris-Saclay, Thiverval-Grignon, France
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany, Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany, Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany
Max Planck Institute for Terrestrial Microbiology, Marburg, Germany

Due to their economic relevance, the study of plant pathogen interactions is of importance. However, elucidating these interactions and their underlying molecular mechanisms remains challenging since both host and pathogen need to be fully genetically accessible organisms. Here we present milestones in the establishment of a new biotrophic model pathosystem: Ustilago bromivora and Brachypodium sp. We provide a complete toolset, including an annotated fungal genome and methods for genetic manipulation of the fungus and its host plant. This toolset will enable researchers to easily study biotrophic interactions at the molecular level on both the pathogen and the host side. Moreover, our research on the fungal life cycle revealed a mating type bias phenomenon. U. bromivora harbors a haplo-lethal allele that is linked to one mating type region. As a result, the identified mating type bias strongly promotes inbreeding, which we consider to be a potential speciation driver.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1331828

Alternate ID(s):: OSTI ID: 1331830; OSTI ID: 1628862

Journal Information:: eLife, Journal Name: eLife Vol. 5; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 35 works

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Target the core: durable plant resistance against filamentous plant pathogens through effector recognition Depotter, Jasper R. L.; Doehlemann, Gunther Pest Management Science, Vol. 76, Issue 2 https://doi.org/10.1002/ps.5677	journal	August 2019
RNAi is a critical determinant of centromere evolution in closely related fungi Yadav, Vikas; Sun, Sheng; Billmyre, R. Blake Proceedings of the National Academy of Sciences, Vol. 115, Issue 12 https://doi.org/10.1073/pnas.1713725115	journal	March 2018
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Smut infection of perennial hosts: the genome and the transcriptome of the Brassicaceae smut fungus Thecaphora thlaspeos reveal functionally conserved and novel effectors Courville, Kaitlyn J.; Frantzeskakis, Lamprinos; Gul, Summia New Phytologist, Vol. 222, Issue 3 https://doi.org/10.1111/nph.15692	journal	February 2019
Cell Biology of Hyphal Growth Steinberg, Gero; Peñalva, Miguel A.; Riquelme, Meritxell The Fungal Kingdom https://doi.org/10.1128/9781555819583.ch11	book	September 2017
Transcripts and tumors: regulatory and metabolic programming during biotrophic phytopathogenesis Schmitz, Lara; McCotter, Sean; Kretschmer, Matthias F1000Research, Vol. 7 https://doi.org/10.12688/f1000research.16404.1	journal	January 2018
Fungal genome and mating system transitions facilitated by chromosomal translocations involving intercentromeric recombination Sun, Sheng; Yadav, Vikas; Billmyre, R. Blake PLOS Biology, Vol. 15, Issue 8 https://doi.org/10.1371/journal.pbio.2002527	journal	August 2017

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