skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Final Technical Report: Role of Methanotrophs in Metal Mobilization, Metal Immobilization and Mineral Weathering: Effects on the In Situ Microbial Community and the Sustainability of Subsurface Water Systems

Abstract

Activities from this DOE sponsored project can be divided into four broad areas: (1) investigations into the potential of methanobactin, a biogenic metal-binding agent produced by methanotrophs, in mitigating mercury toxicity; (2) elucidation of the genetic basis for methanobactin synthesis from methanotrophs; (3) examination of differential gene expression of M. trichosporium OB3b when grown in the presence of varying amounts of copper and/or cerium, and (4) collection and characterization of soil cores from Savannah River Test Site to determine the ubiquity of methanobactin producing methanotrophs. From these efforts, we have conclusively shown that methanobactin can strongly bind mercury as Hg[II], and in so doing significantly reduce the toxicity of this metal to microbes. Further, we have deduced the genetic basis of methanobactin production in methanotrophs, enabling us to construct mutants such that we can now ascribe function to different genes as well as propose a pathway for methanobactin biosynthesis. We have also clear evidence that copper and cerium (as an example of a rare earth element) dramatically affect gene expression in methanotrophs, and thus have an important impact on the activity and application of these microbes to a variety of environmental and industrial issues. Finally, we successfully isolated one methanotrophmore » from the deep subsurface of the Savannah River Test Site and characterized the ability of different forms of methanobactin to mobilize copper and mercury from these soils.« less

Authors:
ORCiD logo [1];  [2]
  1. Univ. of Michigan, Ann Arbor, MI (United States)
  2. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Univ. of Michigan, Ann Arbor, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1330987
Report Number(s):
DOE-Michigan-SC0006630
DOE Contract Number:  
sc0006630
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; methanotrophy; copper; methanobactin; mercury; rare earth elements

Citation Formats

Semrau, Jeremy D., and DiSpirito, Alan A. Final Technical Report: Role of Methanotrophs in Metal Mobilization, Metal Immobilization and Mineral Weathering: Effects on the In Situ Microbial Community and the Sustainability of Subsurface Water Systems. United States: N. p., 2016. Web. doi:10.2172/1330987.
Semrau, Jeremy D., & DiSpirito, Alan A. Final Technical Report: Role of Methanotrophs in Metal Mobilization, Metal Immobilization and Mineral Weathering: Effects on the In Situ Microbial Community and the Sustainability of Subsurface Water Systems. United States. doi:10.2172/1330987.
Semrau, Jeremy D., and DiSpirito, Alan A. Sun . "Final Technical Report: Role of Methanotrophs in Metal Mobilization, Metal Immobilization and Mineral Weathering: Effects on the In Situ Microbial Community and the Sustainability of Subsurface Water Systems". United States. doi:10.2172/1330987. https://www.osti.gov/servlets/purl/1330987.
@article{osti_1330987,
title = {Final Technical Report: Role of Methanotrophs in Metal Mobilization, Metal Immobilization and Mineral Weathering: Effects on the In Situ Microbial Community and the Sustainability of Subsurface Water Systems},
author = {Semrau, Jeremy D. and DiSpirito, Alan A.},
abstractNote = {Activities from this DOE sponsored project can be divided into four broad areas: (1) investigations into the potential of methanobactin, a biogenic metal-binding agent produced by methanotrophs, in mitigating mercury toxicity; (2) elucidation of the genetic basis for methanobactin synthesis from methanotrophs; (3) examination of differential gene expression of M. trichosporium OB3b when grown in the presence of varying amounts of copper and/or cerium, and (4) collection and characterization of soil cores from Savannah River Test Site to determine the ubiquity of methanobactin producing methanotrophs. From these efforts, we have conclusively shown that methanobactin can strongly bind mercury as Hg[II], and in so doing significantly reduce the toxicity of this metal to microbes. Further, we have deduced the genetic basis of methanobactin production in methanotrophs, enabling us to construct mutants such that we can now ascribe function to different genes as well as propose a pathway for methanobactin biosynthesis. We have also clear evidence that copper and cerium (as an example of a rare earth element) dramatically affect gene expression in methanotrophs, and thus have an important impact on the activity and application of these microbes to a variety of environmental and industrial issues. Finally, we successfully isolated one methanotroph from the deep subsurface of the Savannah River Test Site and characterized the ability of different forms of methanobactin to mobilize copper and mercury from these soils.},
doi = {10.2172/1330987},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {11}
}