The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction
Abstract
The HIV-1 accessory protein Vpr is required for efficient viral infection of macrophages and promotion of viral replication in T cells. Vpr's biological activities are closely linked to the interaction with human DCAF1, a cellular substrate receptor of the Cullin4–RING E3 ubiquitin ligase (CRL4) of the host ubiquitin–proteasome-mediated protein degradation pathway. The molecular details of how Vpr usurps the protein degradation pathway have not been delineated. Here we present the crystal structure of the DDB1–DCAF1–HIV-1–Vpr–uracil-DNA glycosylase (UNG2) complex. The structure reveals how Vpr engages with DCAF1, creating a binding interface for UNG2 recruitment in a manner distinct from the recruitment of SAMHD1 by Vpx proteins. Vpr and Vpx use similar N-terminal and helical regions to bind the substrate receptor, whereas different regions target the specific cellular substrates. In conclusion, Vpr uses molecular mimicry of DNA by a variable loop for specific recruitment of the UNG2 substrate.
- Authors:
-
- Univ. of Pittsburgh, PA (United States)
- Stanford Univ., Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Inst. of Health; National Inst. of General Medical Sciences
- OSTI Identifier:
- 1330269
- Grant/Contract Number:
- AC02-76SF00515; P41GM103393; P50GM082251
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Nature Structural & Molecular Biology
- Additional Journal Information:
- Journal Volume: 23; Journal Issue: 10; Journal ID: ISSN 1545-9993
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; virology; X-ray crystallography
Citation Formats
Wu, Ying, Zhou, Xiaohong, Barnes, Christopher O., DeLucia, Maria, Cohen, Aina E., Gronenborn, Angela M., Ahn, Jinwoo, and Calero, Guillermo. The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction. United States: N. p., 2016.
Web. doi:10.1038/nsmb.3284.
Wu, Ying, Zhou, Xiaohong, Barnes, Christopher O., DeLucia, Maria, Cohen, Aina E., Gronenborn, Angela M., Ahn, Jinwoo, & Calero, Guillermo. The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction. United States. https://doi.org/10.1038/nsmb.3284
Wu, Ying, Zhou, Xiaohong, Barnes, Christopher O., DeLucia, Maria, Cohen, Aina E., Gronenborn, Angela M., Ahn, Jinwoo, and Calero, Guillermo. 2016.
"The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction". United States. https://doi.org/10.1038/nsmb.3284. https://www.osti.gov/servlets/purl/1330269.
@article{osti_1330269,
title = {The DDB1–DCAF1–Vpr–UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction},
author = {Wu, Ying and Zhou, Xiaohong and Barnes, Christopher O. and DeLucia, Maria and Cohen, Aina E. and Gronenborn, Angela M. and Ahn, Jinwoo and Calero, Guillermo},
abstractNote = {The HIV-1 accessory protein Vpr is required for efficient viral infection of macrophages and promotion of viral replication in T cells. Vpr's biological activities are closely linked to the interaction with human DCAF1, a cellular substrate receptor of the Cullin4–RING E3 ubiquitin ligase (CRL4) of the host ubiquitin–proteasome-mediated protein degradation pathway. The molecular details of how Vpr usurps the protein degradation pathway have not been delineated. Here we present the crystal structure of the DDB1–DCAF1–HIV-1–Vpr–uracil-DNA glycosylase (UNG2) complex. The structure reveals how Vpr engages with DCAF1, creating a binding interface for UNG2 recruitment in a manner distinct from the recruitment of SAMHD1 by Vpx proteins. Vpr and Vpx use similar N-terminal and helical regions to bind the substrate receptor, whereas different regions target the specific cellular substrates. In conclusion, Vpr uses molecular mimicry of DNA by a variable loop for specific recruitment of the UNG2 substrate.},
doi = {10.1038/nsmb.3284},
url = {https://www.osti.gov/biblio/1330269},
journal = {Nature Structural & Molecular Biology},
issn = {1545-9993},
number = 10,
volume = 23,
place = {United States},
year = {Mon Aug 29 00:00:00 EDT 2016},
month = {Mon Aug 29 00:00:00 EDT 2016}
}
Web of Science
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