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Title: A thioacidolysis method tailored for higher‐throughput quantitative analysis of lignin monomers

Journal Article · · Biotechnology Journal
 [1];  [2];  [1];  [1];  [2];  [2];  [3];  [3];  [1]
  1. Bioenergy Science Center Golden CO USA, National Bioenergy Center, National Renewable Energy Laboratory Golden CO USA
  2. Great Lakes BioEnergy Research Center Michigan State University East Lansing MI USA
  3. Wisconsin Bioenergy Initiative University of Wisconsin Madison WI USA
+ Show Author Affiliations

Thioacidolysis is a method used to measure the relative content of lignin monomers bound by β-O-4 linkages. Current thioacidolysis methods are low-throughput as they require tedious steps for reaction product concentration prior to analysis using standard GC methods. A quantitative thioacidolysis method that is accessible with general laboratory equipment and uses a non-chlorinated organic solvent and is tailored for higher-throughput analysis is reported. The method utilizes lignin arylglycerol monomer standards for calibration, requires 1-2 mg of biomass per assay and has been quantified using fast-GC techniques including a Low Thermal Mass Modular Accelerated Column Heater (LTM MACH). Cumbersome steps, including standard purification, sample concentrating and drying have been eliminated to help aid in consecutive day-to-day analyses needed to sustain a high sample throughput for large screening experiments without the loss of quantitation accuracy. As a result, the method reported in this manuscript has been quantitatively validated against a commonly used thioacidolysis method and across two different research sites with three common biomass varieties to represent hardwoods, softwoods, and grasses.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
DOE Office of Science BER DE‐FC02‐07ER64494; No. DE‐AC36‐08‐GO28308; AC36-08GO28308
OSTI ID:
1324472
Alternate ID(s):
OSTI ID: 1328999; OSTI ID: 1345672
Report Number(s):
NREL/JA-5100-65297
Journal Information:
Biotechnology Journal, Journal Name: Biotechnology Journal Vol. 11 Journal Issue: 10; ISSN 1860-6768
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
Germany
Language:
English
Citation Metrics:
Cited by: 30 works
Citation information provided by
Web of Science

References (17)

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Transgenic switchgrass ( Panicum virgatum L.) biomass is increased by overexpression of switchgrass sucrose synthase ( PvSUS1 ) journal November 2014
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Thioacidolysis of Spruce Lignin: GC-MS Analysis of the Main Dimers Recovered After Raney Nickel Desulphuration journal January 1991
Syntheses of Lignin-Derived Thioacidolysis Monomers and Their Uses as Quantitation Standards journal January 2012
Microscale thioacidolysis method for the rapid analysis of ^|^#946;-O-4 substructures in lignin journal January 2012
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09 BIOMASS FUELS
cell wall structure
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thioacidolysis