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Title: Intein-modified enzymes, their production and industrial applications

Abstract

A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
Agrivida, Inc. Medford, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1328698
Patent Number(s):
9,464,333
Application Number:
13/508,234
Assignee:
Agrivida, Inc. (Medford, MA
DOE Contract Number:  
AR0000042
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Nov 05
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Apgar, James, Lessard, Philip, Raab, Michael R., Shen, Binzhang, Lazar, Gabor, and de la Vega, Humberto. Intein-modified enzymes, their production and industrial applications. United States: N. p., 2016. Web.
Apgar, James, Lessard, Philip, Raab, Michael R., Shen, Binzhang, Lazar, Gabor, & de la Vega, Humberto. Intein-modified enzymes, their production and industrial applications. United States.
Apgar, James, Lessard, Philip, Raab, Michael R., Shen, Binzhang, Lazar, Gabor, and de la Vega, Humberto. 2016. "Intein-modified enzymes, their production and industrial applications". United States. https://www.osti.gov/servlets/purl/1328698.
@article{osti_1328698,
title = {Intein-modified enzymes, their production and industrial applications},
author = {Apgar, James and Lessard, Philip and Raab, Michael R. and Shen, Binzhang and Lazar, Gabor and de la Vega, Humberto},
abstractNote = {A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.},
doi = {},
url = {https://www.osti.gov/biblio/1328698}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {10}
}

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